A fast sequential injection analysis system for the simultaneous determination of ammonia and phosphate
Research output: Journal contributions › Journal articles › Research › peer-review
Standard
In: Microchimica acta, Vol. 154, No. 1-2, 04.2006, p. 31-38.
Research output: Journal contributions › Journal articles › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - A fast sequential injection analysis system for the simultaneous determination of ammonia and phosphate
AU - Frank, Carsten
AU - Schroeder, Friedhelm
AU - Ebinghaus, Ralf
AU - Ruck, Wolfgang
N1 - Parallel als Online-Ausg. erschienen unter der Adresse http://dx.doi.org/10.1007/s00604-006-0496-y
PY - 2006/4
Y1 - 2006/4
N2 - A flow system is described that is based on sequential injection analysis (SIA) and is suitable for the fast determination of ammonia and phosphate in river and marine waters. It is applicable to nutrient mapping in inhomogeneous coastal areas like the Wadden Sea, and was optimised on several cruises on the North Sea. The high sample throughput of 120 samples per analyte per hour and the low reagent consumption (ammonia: 62.5 μL; phosphate: 60 μL) were achieved using a home made programme written in the python programming language. The determination of free reactive phosphate is based on the reaction of phosphate with acidic molybdate to phosphomolybdate which forms nonfluorescent ion pairs with rhodamine 6G. The remaining rhodamine fluorescence is detected at 550 nm with an excitation at 470 nm. Ammonia is determined with the help of o-phthaldialdehyde and sodium sulfite. At 85°C and a reaction time of at least one minute a fluorescent species (exc. 365nm, em. 425nm) is formed. The detection limits are (3σ) 0.3 μmol L -1 for phosphate and 1 μmol L -1 for ammonia.
AB - A flow system is described that is based on sequential injection analysis (SIA) and is suitable for the fast determination of ammonia and phosphate in river and marine waters. It is applicable to nutrient mapping in inhomogeneous coastal areas like the Wadden Sea, and was optimised on several cruises on the North Sea. The high sample throughput of 120 samples per analyte per hour and the low reagent consumption (ammonia: 62.5 μL; phosphate: 60 μL) were achieved using a home made programme written in the python programming language. The determination of free reactive phosphate is based on the reaction of phosphate with acidic molybdate to phosphomolybdate which forms nonfluorescent ion pairs with rhodamine 6G. The remaining rhodamine fluorescence is detected at 550 nm with an excitation at 470 nm. Ammonia is determined with the help of o-phthaldialdehyde and sodium sulfite. At 85°C and a reaction time of at least one minute a fluorescent species (exc. 365nm, em. 425nm) is formed. The detection limits are (3σ) 0.3 μmol L -1 for phosphate and 1 μmol L -1 for ammonia.
KW - Chemistry
KW - Phosphate
KW - ammonia
KW - sequential injection analysis
KW - marine waters
KW - Phosphate
KW - ammonia
KW - sequential injection analysis
KW - marine waters
UR - http://www.scopus.com/inward/record.url?scp=33645997667&partnerID=8YFLogxK
U2 - 10.1007/s00604-006-0496-y
DO - 10.1007/s00604-006-0496-y
M3 - Journal articles
VL - 154
SP - 31
EP - 38
JO - Microchimica acta
JF - Microchimica acta
SN - 0026-3672
IS - 1-2
ER -