Fast, Fully Automated Analysis of Voriconazole from Serum by LC-LC-ESI-MS-MS with Parallel Column-Switching Technique

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschungbegutachtet

Authors

Voriconazole is a novel broad-spectrum antifungal agent. We developed an on-line LC-LC-MS-MS method for fully automated and direct analysis of voriconazole in raw human serum. After injection of human serum size-selective sample fractionation and analyte extraction was achieved using an extraction column (25 mm × 4 mm) packed with a restricted access material (RAM, LiChrospher® ADS C 8, 25 μm). On-line transfer of voriconazole from the extraction column was followed by chromatography separation on a C 18 column. Detection was done by ESI-MS-MS. The total analysis time was 13 min, managed by parallel extraction and chromatographic separation. This LC-MS assay was fully validated. The lower limit of quantification was 0.05 μg/ml. The automated inline extraction of voriconazole described here eliminates the need for difficult and time-consuming sample pre-treatment. Other advantages of the new method are that only a small quantity (5 μl) of serum is needed and that the method is very specific. © 2004 Elsevier B.V. All rights reserved.
OriginalspracheEnglisch
ZeitschriftJournal of Chromatography B
Jahrgang814
Ausgabenummer2
Seiten (von - bis)361-367
Anzahl der Seiten7
DOIs
PublikationsstatusErschienen - 25.01.2005
Extern publiziertJa

    Fachgebiete

  • Chemie - Voriconazole , Column-switching, LC–LC–ESI-MS–MS, Serum, On-line sample preparation

DOI

Zuletzt angesehen

Publikationen

  1. Computational modeling of amorphous polymers
  2. Dynamic Lot Size Optimization with Reinforcement Learning
  3. The delay vector variance method and the recurrence quantification analysis of energy markets
  4. Dispatching rule selection with Gaussian processes
  5. Analysis of long-term statistical data of cobalt flows in the EU
  6. Computing regression statistics from grouped data
  7. Image compression based on periodic principal components
  8. Gaussian processes for dispatching rule selection in production scheduling
  9. Digging into the roots
  10. Gain Adaptation in Sliding Mode Control Using Model Predictive Control and Disturbance Compensation with Application to Actuators
  11. Knowledge Graph Question Answering and Large Language Models
  12. Challenge-oriented policy making and innovation systems theory: reconsidering systemic instruments
  13. Faulty Process Detection Using Machine Learning Techniques
  14. What can conservation strategies learn from the ecosystem services approach?
  15. A Class of Simple Stochastic Online Bin Packing Algorithms
  16. Constraints are the solution, not the problem
  17. Development and evaluation of a training program for dialysis nurses - An intervention study
  18. Overcoming Multi-legacy Application Challenges through Building Dynamic Capabilities for Low-Code Adoption
  19. Positioning Improvement for a Laser Scanning System using cSORPD control
  20. Extending talk on a prescribed discussion topic in a learner-native speaker eTandem learning task
  21. A Multimethod Latent State-Trait Model for Structurally Different and Interchangeable Methods
  22. Handicaps in job assignment
  23. Covert and overt automatic imitation are correlated
  24. Towards an open question answering architecture
  25. Knowledge transfer during the integration of knowledge-intensive acquisitions