Optimization and Validation of an LC Method for the Determination of Cefdinir in Dosage Form and Human Urine

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  • Ghada M. Hadad
  • Samy Emara
  • Waleed M. M. Mahmoud
A simple and reliable liquid chromatographic method has been developed and validated for the determination of cefdinir in human urine and capsule samples. A chromatographic separation was achieved on a C18 column using a mobile phase consisting of potassium dihydrogen phosphate (10 mM, pH 4.5)–acetonitrile (90:10, v/v). Quantitation was achieved with UV detection at 285 nm, based on peak area with linear calibration curve at a concentration range of 0.7–39 µg mL−1. This method was successfully applied for the establishment of an urinary excretion pattern after oral dose.
Original languageEnglish
Issue number11/12
Pages (from-to)1593–1598
Number of pages6
Publication statusPublished - 12.2009

    Research areas

  • Chemistry - Column liquid chromatography, Urinary excretion pattern, Cefdinir