Optimization and validation of an HPLC-UV method for determination of tranexamic acid in a dosage form and in human urine.

Research output: Journal contributionsJournal articlesResearchpeer-review

Authors

  • Ghada M. Hadad
  • Alaa El-Gindy
  • Waleed M. M. Mahmoud
A simple and reliable high-performance liquid chromatographic method with UV detection at 245 nm has been developed and validated for determination of tranexamic acid (TA) in a dosage form and in human urine. Before injection samples were derivatized with phenyl isothiocyanate (PITC). The reaction temperature, reaction time, and concentration of PITC used for derivatization were optimized. Chromatographic separation was on a C18 column with a 65:35 (v/v) mixture of 10 mM phosphate buffer, pH 3.6, and acetonitrile as mobile phase. Heptaminol hydrochloride was used as internal standard.
Original languageEnglish
JournalChromatographia
Volume66
Issue number5-6
Pages (from-to)311-317
Number of pages7
ISSN0009-5893
DOIs
Publication statusPublished - 09.2007

    Research areas

  • Chemistry - Column liquid chromatography, Urinary excretion, Tranexamic acid

Recently viewed

Publications

  1. Ludic interfaces
  2. A Sliding Mode Control with a Bang-Bang Observer for Detection of Particle Pollution
  3. Toward Data-Driven Analyses of Electronic Text Books
  4. Sensor concept for solving the direct kinematics problem of the Stewart-Gough platform
  5. Imaginary practices as the nexus between continuity and disruptive change
  6. Finite element based determination and optimization of seam weld positions in porthole die extrusion of double hollow profile with asymmetric cross section
  7. How can employment relations in global value networks be managed towards social responsibility?
  8. Reduction of capital tie up for assembly processes
  9. The Politics of (Non)Knowledge in the (Un)Making of Migration
  10. A comparison between private and public access rules to bottlenecks - experiences and expectations from telecommunication and energy
  11. Article 11 Formal Validity
  12. Toward a gecko-inspired, climbing soft robot
  13. Microstructure evolution of Mg-11Gd-4.5Y-1Nd-1.5Zn-0.5Zr (wt%) alloy during deformation and its effect on strengthening
  14. Structure and Organization of Product Development Projects
  15. Review of transit data sources
  16. Archives
  17. Development of a Questionnaire Assessing Discomfort – The Disco-Scale
  18. Great ape cognition is structured by stable cognitive abilities and predicted by developmental conditions
  19. IFIP WG 13.5 workshop on resilience, reliability, safety and human error in system development
  20. Narrative consistency across replays of pro-social interactive digital narratives
  21. Optimal control strategies for PMSM with a decoupling super twisting SMC and inductance estimation in the presence of saturation
  22. Whose Change is it, Anyway?
  23. Toward a lifespan metric of reading fluency
  24. Adaptor device for transmitting e.g. blood pressure data of human body from blood pressure measuring device of data communication system to e.g. personal computer, has controller for controlling transmission of data to communication module
  25. Discourse of ‘Self’ and ‘Other’ in Newspaper Editorials on Insecurity in Nigeria
  26. Belowground top-down and aboveground bottom-up effects structure multitrophic community relationships in a biodiverse forest
  27. Influence of ageing treatment on microstructure, mechanical and bio-corrosion properties of Mg-Dy alloys
  28. Residual stresses in continuously reinforced composite profiles with symmetric cross sections
  29. Building Scene and Cultural Memory in the Weser Hills.
  30. Developing pathways for energy storage in the UK using a coevolutionary framework
  31. Proceedings SMC 2016
  32. The efficiency of German public theaters: a stochastic frontier analysis approach
  33. An Adaptive Resonance Regulator Design for Motion Control of Intake Valves in Camless Engine Systems
  34. Supporting non-hierarchical supply chain networks in the electronics industry
  35. Report on the relative strengths and weaknesses of the United States in PISA 2012 mathematics

Press / Media

  1. Die Rechnung, bitte