Optimization and validation of an HPLC-UV method for determination of tranexamic acid in a dosage form and in human urine.

Research output: Journal contributionsJournal articlesResearchpeer-review

Authors

  • Ghada M. Hadad
  • Alaa El-Gindy
  • Waleed M. M. Mahmoud
A simple and reliable high-performance liquid chromatographic method with UV detection at 245 nm has been developed and validated for determination of tranexamic acid (TA) in a dosage form and in human urine. Before injection samples were derivatized with phenyl isothiocyanate (PITC). The reaction temperature, reaction time, and concentration of PITC used for derivatization were optimized. Chromatographic separation was on a C18 column with a 65:35 (v/v) mixture of 10 mM phosphate buffer, pH 3.6, and acetonitrile as mobile phase. Heptaminol hydrochloride was used as internal standard.
Original languageEnglish
JournalChromatographia
Volume66
Issue number5-6
Pages (from-to)311-317
Number of pages7
ISSN0009-5893
DOIs
Publication statusPublished - 09.2007

    Research areas

  • Chemistry - Column liquid chromatography, Urinary excretion, Tranexamic acid