Modification of the umu-assay (ISO 13829) accounting for cytotoxicity in genotoxicity assessment: A preliminary study

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Modification of the umu-assay (ISO 13829) accounting for cytotoxicity in genotoxicity assessment: A preliminary study. / Toolaram, Anju Priya; Gutiérrez, Iris Raquel; Ahlf, Wolfgang.
In: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 747, No. 2, 18.09.2012, p. 190-196.

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@article{16b9bcb8c0e9441a99506c7715345565,
title = "Modification of the umu-assay (ISO 13829) accounting for cytotoxicity in genotoxicity assessment: A preliminary study",
abstract = "In this study, the umu-assay (ISO 13829) was modified by addition of the resazurin-reduction assay to assess the cytotoxic potential of toxins. The aim was to develop a test system that was capable of examining both genotoxicity and cytotoxicity on the basis of the metabolic health of the cells so as to provide a better assessment of the negative influence of toxic effects on the evaluation of genotoxicity. The test was established and validated with mitomycin C (MMC), 1,3-dinitropyrene (1,3-DNP), 4-nitroquinoline-1-oxide (4-NQO) and chloramphenicol (CHL) as toxins with known responses in the umu-assay. The results indicate that the modified umu-assay was able to reveal genotoxic responses towards MMC, 1,3-DNP and 4-NQO up to 1 μg/ml. Further, the assay was able to determine the cytotoxicity of CHL, MMC and 4-NQO. Hence, the modified umu-assay was proven to be a sensitive test for determining the cytotoxicity and genotoxicity of toxins, with results that were comparable with literature data. Moreover, the modified umu-assay showed that cytotoxic concentrations that cause >50% inhibition of dehydrogenase activity (DHA) can result in unreliable computation of genotoxicity.",
keywords = "Chemistry, umu-Assay, Cytotoxicity, Genotoxicity, Resazurin-reduction assay, Sustainability Science",
author = "Toolaram, {Anju Priya} and Guti{\'e}rrez, {Iris Raquel} and Wolfgang Ahlf",
year = "2012",
month = sep,
day = "18",
doi = "10.1016/j.mrgentox.2012.05.010",
language = "English",
volume = "747",
pages = "190--196",
journal = "Mutation Research/Genetic Toxicology and Environmental Mutagenesis",
issn = "1383-5718",
publisher = "Elsevier B.V.",
number = "2",

}

RIS

TY - JOUR

T1 - Modification of the umu-assay (ISO 13829) accounting for cytotoxicity in genotoxicity assessment: A preliminary study

AU - Toolaram, Anju Priya

AU - Gutiérrez, Iris Raquel

AU - Ahlf, Wolfgang

PY - 2012/9/18

Y1 - 2012/9/18

N2 - In this study, the umu-assay (ISO 13829) was modified by addition of the resazurin-reduction assay to assess the cytotoxic potential of toxins. The aim was to develop a test system that was capable of examining both genotoxicity and cytotoxicity on the basis of the metabolic health of the cells so as to provide a better assessment of the negative influence of toxic effects on the evaluation of genotoxicity. The test was established and validated with mitomycin C (MMC), 1,3-dinitropyrene (1,3-DNP), 4-nitroquinoline-1-oxide (4-NQO) and chloramphenicol (CHL) as toxins with known responses in the umu-assay. The results indicate that the modified umu-assay was able to reveal genotoxic responses towards MMC, 1,3-DNP and 4-NQO up to 1 μg/ml. Further, the assay was able to determine the cytotoxicity of CHL, MMC and 4-NQO. Hence, the modified umu-assay was proven to be a sensitive test for determining the cytotoxicity and genotoxicity of toxins, with results that were comparable with literature data. Moreover, the modified umu-assay showed that cytotoxic concentrations that cause >50% inhibition of dehydrogenase activity (DHA) can result in unreliable computation of genotoxicity.

AB - In this study, the umu-assay (ISO 13829) was modified by addition of the resazurin-reduction assay to assess the cytotoxic potential of toxins. The aim was to develop a test system that was capable of examining both genotoxicity and cytotoxicity on the basis of the metabolic health of the cells so as to provide a better assessment of the negative influence of toxic effects on the evaluation of genotoxicity. The test was established and validated with mitomycin C (MMC), 1,3-dinitropyrene (1,3-DNP), 4-nitroquinoline-1-oxide (4-NQO) and chloramphenicol (CHL) as toxins with known responses in the umu-assay. The results indicate that the modified umu-assay was able to reveal genotoxic responses towards MMC, 1,3-DNP and 4-NQO up to 1 μg/ml. Further, the assay was able to determine the cytotoxicity of CHL, MMC and 4-NQO. Hence, the modified umu-assay was proven to be a sensitive test for determining the cytotoxicity and genotoxicity of toxins, with results that were comparable with literature data. Moreover, the modified umu-assay showed that cytotoxic concentrations that cause >50% inhibition of dehydrogenase activity (DHA) can result in unreliable computation of genotoxicity.

KW - Chemistry

KW - umu-Assay

KW - Cytotoxicity

KW - Genotoxicity

KW - Resazurin-reduction assay

KW - Sustainability Science

UR - http://www.scopus.com/inward/record.url?scp=84863779946&partnerID=8YFLogxK

U2 - 10.1016/j.mrgentox.2012.05.010

DO - 10.1016/j.mrgentox.2012.05.010

M3 - Journal articles

C2 - 22659122

VL - 747

SP - 190

EP - 196

JO - Mutation Research/Genetic Toxicology and Environmental Mutagenesis

JF - Mutation Research/Genetic Toxicology and Environmental Mutagenesis

SN - 1383-5718

SN - 1879-3592

IS - 2

ER -