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Modification of the umu-assay (ISO 13829) accounting for cytotoxicity in genotoxicity assessment: A preliminary study

Research output: Journal contributions › Journal articles › Research

Standard

Modification of the umu-assay (ISO 13829) accounting for cytotoxicity in genotoxicity assessment: A preliminary study. / Toolaram, Anju Priya; Gutiérrez, Iris Raquel; Ahlf, Wolfgang.
In: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 747, No. 2, 18.09.2012, p. 190-196.

Research output: Journal contributions › Journal articles › Research

Harvard

Toolaram, AP, Gutiérrez, IR & Ahlf, W 2012, 'Modification of the umu-assay (ISO 13829) accounting for cytotoxicity in genotoxicity assessment: A preliminary study', Mutation Research/Genetic Toxicology and Environmental Mutagenesis, vol. 747, no. 2, pp. 190-196. https://doi.org/10.1016/j.mrgentox.2012.05.010

APA

Toolaram, A. P., Gutiérrez, I. R., & Ahlf, W. (2012). Modification of the umu-assay (ISO 13829) accounting for cytotoxicity in genotoxicity assessment: A preliminary study. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 747(2), 190-196. https://doi.org/10.1016/j.mrgentox.2012.05.010

Vancouver

Toolaram AP, Gutiérrez IR, Ahlf W. Modification of the umu-assay (ISO 13829) accounting for cytotoxicity in genotoxicity assessment: A preliminary study. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2012 Sept 18;747(2):190-196. doi: 10.1016/j.mrgentox.2012.05.010

Bibtex

@article{16b9bcb8c0e9441a99506c7715345565,
title = "Modification of the umu-assay (ISO 13829) accounting for cytotoxicity in genotoxicity assessment: A preliminary study",
abstract = "In this study, the umu-assay (ISO 13829) was modified by addition of the resazurin-reduction assay to assess the cytotoxic potential of toxins. The aim was to develop a test system that was capable of examining both genotoxicity and cytotoxicity on the basis of the metabolic health of the cells so as to provide a better assessment of the negative influence of toxic effects on the evaluation of genotoxicity. The test was established and validated with mitomycin C (MMC), 1,3-dinitropyrene (1,3-DNP), 4-nitroquinoline-1-oxide (4-NQO) and chloramphenicol (CHL) as toxins with known responses in the umu-assay. The results indicate that the modified umu-assay was able to reveal genotoxic responses towards MMC, 1,3-DNP and 4-NQO up to 1 μg/ml. Further, the assay was able to determine the cytotoxicity of CHL, MMC and 4-NQO. Hence, the modified umu-assay was proven to be a sensitive test for determining the cytotoxicity and genotoxicity of toxins, with results that were comparable with literature data. Moreover, the modified umu-assay showed that cytotoxic concentrations that cause >50% inhibition of dehydrogenase activity (DHA) can result in unreliable computation of genotoxicity.",
keywords = "Chemistry, umu-Assay, Cytotoxicity, Genotoxicity, Resazurin-reduction assay, Sustainability Science",
author = "Toolaram, {Anju Priya} and Guti{\'e}rrez, {Iris Raquel} and Wolfgang Ahlf",
year = "2012",
month = sep,
day = "18",
doi = "10.1016/j.mrgentox.2012.05.010",
language = "English",
volume = "747",
pages = "190--196",
journal = "Mutation Research/Genetic Toxicology and Environmental Mutagenesis",
issn = "1383-5718",
publisher = "Elsevier B.V.",
number = "2",

}

RIS

TY - JOUR

T1 - Modification of the umu-assay (ISO 13829) accounting for cytotoxicity in genotoxicity assessment: A preliminary study

AU - Toolaram, Anju Priya

AU - Gutiérrez, Iris Raquel

AU - Ahlf, Wolfgang

PY - 2012/9/18

Y1 - 2012/9/18

N2 - In this study, the umu-assay (ISO 13829) was modified by addition of the resazurin-reduction assay to assess the cytotoxic potential of toxins. The aim was to develop a test system that was capable of examining both genotoxicity and cytotoxicity on the basis of the metabolic health of the cells so as to provide a better assessment of the negative influence of toxic effects on the evaluation of genotoxicity. The test was established and validated with mitomycin C (MMC), 1,3-dinitropyrene (1,3-DNP), 4-nitroquinoline-1-oxide (4-NQO) and chloramphenicol (CHL) as toxins with known responses in the umu-assay. The results indicate that the modified umu-assay was able to reveal genotoxic responses towards MMC, 1,3-DNP and 4-NQO up to 1 μg/ml. Further, the assay was able to determine the cytotoxicity of CHL, MMC and 4-NQO. Hence, the modified umu-assay was proven to be a sensitive test for determining the cytotoxicity and genotoxicity of toxins, with results that were comparable with literature data. Moreover, the modified umu-assay showed that cytotoxic concentrations that cause >50% inhibition of dehydrogenase activity (DHA) can result in unreliable computation of genotoxicity.

AB - In this study, the umu-assay (ISO 13829) was modified by addition of the resazurin-reduction assay to assess the cytotoxic potential of toxins. The aim was to develop a test system that was capable of examining both genotoxicity and cytotoxicity on the basis of the metabolic health of the cells so as to provide a better assessment of the negative influence of toxic effects on the evaluation of genotoxicity. The test was established and validated with mitomycin C (MMC), 1,3-dinitropyrene (1,3-DNP), 4-nitroquinoline-1-oxide (4-NQO) and chloramphenicol (CHL) as toxins with known responses in the umu-assay. The results indicate that the modified umu-assay was able to reveal genotoxic responses towards MMC, 1,3-DNP and 4-NQO up to 1 μg/ml. Further, the assay was able to determine the cytotoxicity of CHL, MMC and 4-NQO. Hence, the modified umu-assay was proven to be a sensitive test for determining the cytotoxicity and genotoxicity of toxins, with results that were comparable with literature data. Moreover, the modified umu-assay showed that cytotoxic concentrations that cause >50% inhibition of dehydrogenase activity (DHA) can result in unreliable computation of genotoxicity.

KW - Chemistry

KW - umu-Assay

KW - Cytotoxicity

KW - Genotoxicity

KW - Resazurin-reduction assay

KW - Sustainability Science

UR - http://www.scopus.com/inward/record.url?scp=84863779946&partnerID=8YFLogxK

U2 - 10.1016/j.mrgentox.2012.05.010

DO - 10.1016/j.mrgentox.2012.05.010

M3 - Journal articles

C2 - 22659122

VL - 747

SP - 190

EP - 196

JO - Mutation Research/Genetic Toxicology and Environmental Mutagenesis

JF - Mutation Research/Genetic Toxicology and Environmental Mutagenesis

SN - 1383-5718

SN - 1879-3592

IS - 2

ER -

Other publications by the same author(s)

Hazard screening of photo-transformation products from pharmaceuticals: Application to selective β1-blockers atenolol and metoprolol

Toolaram, A. P., Menz, J., Rastogi, T., Leder, C., Kümmerer, K. & Schneider, M., 01.02.2017, In: The Science of The Total Environment. 579, p. 1769-1780 12 p.

Research output: Journal contributions › Journal articles › Research › peer-review

Initial hazard screening for genotoxicity of photo-transformation products of ciprofloxacin by applying a combination of experimental and in-silico testing

Toolaram, A. P., Haddad, T., Leder, C. & Kümmerer, K., 01.04.2016, In: Environmental Pollution. 211, p. 148-156 9 p.

Research output: Journal contributions › Journal articles › Research › peer-review

Transformation products in the water cycle and the unsolved problem of their proactive assessment: A combined in vitro/in silico approach

Menz, J., Toolaram, A. P., Rastogi, T., Leder, C., Olsson, O., Kümmerer, K. & Schneider, M., 01.01.2017, In: Environmental international. 98, p. 171-180 10 p.

Research output: Journal contributions › Journal articles › Research › peer-review

Environmental risk assessment of anti-cancer drugs and their transformation products: a focus on their genotoxicity characterization-state of knowledge and short comings

Toolaram, A. P., Kümmerer, K. & Schneider, M., 01.04.2014, In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 760, p. 18-35 18 p.

Research output: Journal contributions › Scientific review articles › Research

Identification and initial toxicity assessment of Thalidomide and its phototransformation products

Mahmoud, W. M. M., Toolaram, A. P., Menz, J., Leder, C., Schneider, M. & Kümmerer, K., 2013, 14th EuCheMS International Conference on Chemistry and the Environment. European Association for Chemical and Molecular Sciences / Division of Organic Chemistry, p. 366 1 p.

Research output: Contributions to collected editions/works › Published abstract in conference proceedings › Research › peer-review

DOI

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