Geometrical Characterization of Polyethylene Oxide Nanofibers by Atom Force Microscope and Confocal Laser Scanning Microscope

Research output: Contributions to collected editions/worksArticle in conference proceedingsResearch


The aim of our research is the development of a prototype of a Bionanosensor, which can detected the pathogens (virus, bacteria) in enclosed spaces (such as hospitals) in real time. The Polymers nanofibers act as sensing element of the Bionanosensor, therefore is important to investigate the geometrical, mechanical and electrical properties of it. The first objective of our work is to find the optimal electrospinning parameters to produce polymer fibers under 100 nm (nanofibers) and the geometrical characterization of it. These nanofibers are ideal as sensing element of the sensor, since the average size of a Virus is approximately 100 nm.
In this paper we present the geometric properties of the nanofibers of polyethylene oxide (PEO) and the optimal parameters of electrospinning to produce them. We have deposited PEO nanofi-bers over a glass plate and used an Atomic Force Microscope (AFM) and Confocal Laser Scan-ning Microscope (CLSM) to measure their topography. We are focusing on the measurement of the fiber´s diameter and the length. The cross section measurement by AFM reveals a height of 79.8 nm and width of 159.7 nm of the fibers. The electrospinning parameters to produce this fibers were; Voltage: 11KV, distance between electrodes: 20 cm, and Feed Rate: 0,008 ml/min. Fur-thermore we propose a size-correction model for the fiber under 100 nm.
Original languageEnglish
Title of host publicationFiberMad 11, International Conference on Fibrous Products in Medical and Health Care
EditorsPaivi Talvenmaa
Number of pages7
PublisherTampere University of Technology
Publication date30.06.2011
ISBN (print)978-952-15-2607-7
Publication statusPublished - 30.06.2011
Event11th International Conference on Fibrous Products in Medical and Health Care - FiberMad 2011 - Tampere, Finland
Duration: 28.06.201130.06.2011
Conference number: 11