Evaluation of short-term effects of rare earth and other elements used in magnesium alloys on primary cells and cell lines
Research output: Journal contributions › Journal articles › Research › peer-review
Standard
In: Acta Biomaterialia, Vol. 6, No. 5, 05.2010, p. 1834-1842.
Research output: Journal contributions › Journal articles › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Evaluation of short-term effects of rare earth and other elements used in magnesium alloys on primary cells and cell lines
AU - Feyerabend, Frank
AU - Fischer, Janine
AU - Holtz, Jakob
AU - Witte, Frank
AU - Willumeit, Regine
AU - Drücker, Heiko
AU - Vogt, Carla
AU - Hort, Norbert
PY - 2010/5
Y1 - 2010/5
N2 - Degradable magnesium alloys for biomedical application are on the verge of being used clinically. Rare earth elements (REEs) are used to improve the mechanical properties of the alloys, but in more or less undefined mixtures. For some elements of this group, data on toxicity and influence on cells are sparse. Therefore in this study the in vitro cytotoxicity of the elements yttrium (Y), neodymium (Nd), dysprosium (Dy), praseodymium (Pr), gadolinium (Gd), lanthanum (La), cerium (Ce), europium (Eu), lithium (Li) and zirconium (Zr) was evaluated by incubation with the chlorides (10-2000 μM); magnesium (Mg) and calcium (Ca) were tested at higher concentrations (200 and 50 mM, respectively). The influence on viability of human osteosarcoma cell line MG63, human umbilical cord perivascular (HUCPV) cells and mouse macrophages (RAW 264.7) was determined, as well as the induction of apoptosis and the expression of inflammatory factors (TNF-α, IL-1α). Significant differences between the applied cells could be observed. RAW exhibited the highest and HUCPV the lowest sensitivity. La and Ce showed the highest cytotoxicity of the analysed elements. Of the elements with high solubility in magnesium alloys, Gd and Dy seem to be more suitable than Y. The focus of magnesium alloy development for biomedical applications should include most defined alloy compositions with well-known tissue-specific and systemic effects.
AB - Degradable magnesium alloys for biomedical application are on the verge of being used clinically. Rare earth elements (REEs) are used to improve the mechanical properties of the alloys, but in more or less undefined mixtures. For some elements of this group, data on toxicity and influence on cells are sparse. Therefore in this study the in vitro cytotoxicity of the elements yttrium (Y), neodymium (Nd), dysprosium (Dy), praseodymium (Pr), gadolinium (Gd), lanthanum (La), cerium (Ce), europium (Eu), lithium (Li) and zirconium (Zr) was evaluated by incubation with the chlorides (10-2000 μM); magnesium (Mg) and calcium (Ca) were tested at higher concentrations (200 and 50 mM, respectively). The influence on viability of human osteosarcoma cell line MG63, human umbilical cord perivascular (HUCPV) cells and mouse macrophages (RAW 264.7) was determined, as well as the induction of apoptosis and the expression of inflammatory factors (TNF-α, IL-1α). Significant differences between the applied cells could be observed. RAW exhibited the highest and HUCPV the lowest sensitivity. La and Ce showed the highest cytotoxicity of the analysed elements. Of the elements with high solubility in magnesium alloys, Gd and Dy seem to be more suitable than Y. The focus of magnesium alloy development for biomedical applications should include most defined alloy compositions with well-known tissue-specific and systemic effects.
KW - In vitro
KW - Inflammatory response
KW - Magnesium
KW - Rare earth elements
KW - Toxicity
KW - Engineering
UR - http://www.scopus.com/inward/record.url?scp=77954681094&partnerID=8YFLogxK
U2 - 10.1016/j.actbio.2009.09.024
DO - 10.1016/j.actbio.2009.09.024
M3 - Journal articles
C2 - 19800429
AN - SCOPUS:77954681094
VL - 6
SP - 1834
EP - 1842
JO - Acta Biomaterialia
JF - Acta Biomaterialia
SN - 1742-7061
IS - 5
ER -