TY - JOUR

T1 - Evaluation of short-term effects of rare earth and other elements used in magnesium alloys on primary cells and cell lines

AU - Feyerabend, Frank

AU - Fischer, Janine

AU - Holtz, Jakob

AU - Witte, Frank

AU - Willumeit, Regine

AU - Drücker, Heiko

AU - Vogt, Carla

AU - Hort, Norbert

PY - 2010/5

Y1 - 2010/5

N2 - Degradable magnesium alloys for biomedical application are on the verge of being used clinically. Rare earth elements (REEs) are used to improve the mechanical properties of the alloys, but in more or less undefined mixtures. For some elements of this group, data on toxicity and influence on cells are sparse. Therefore in this study the in vitro cytotoxicity of the elements yttrium (Y), neodymium (Nd), dysprosium (Dy), praseodymium (Pr), gadolinium (Gd), lanthanum (La), cerium (Ce), europium (Eu), lithium (Li) and zirconium (Zr) was evaluated by incubation with the chlorides (10-2000 μM); magnesium (Mg) and calcium (Ca) were tested at higher concentrations (200 and 50 mM, respectively). The influence on viability of human osteosarcoma cell line MG63, human umbilical cord perivascular (HUCPV) cells and mouse macrophages (RAW 264.7) was determined, as well as the induction of apoptosis and the expression of inflammatory factors (TNF-α, IL-1α). Significant differences between the applied cells could be observed. RAW exhibited the highest and HUCPV the lowest sensitivity. La and Ce showed the highest cytotoxicity of the analysed elements. Of the elements with high solubility in magnesium alloys, Gd and Dy seem to be more suitable than Y. The focus of magnesium alloy development for biomedical applications should include most defined alloy compositions with well-known tissue-specific and systemic effects.

AB - Degradable magnesium alloys for biomedical application are on the verge of being used clinically. Rare earth elements (REEs) are used to improve the mechanical properties of the alloys, but in more or less undefined mixtures. For some elements of this group, data on toxicity and influence on cells are sparse. Therefore in this study the in vitro cytotoxicity of the elements yttrium (Y), neodymium (Nd), dysprosium (Dy), praseodymium (Pr), gadolinium (Gd), lanthanum (La), cerium (Ce), europium (Eu), lithium (Li) and zirconium (Zr) was evaluated by incubation with the chlorides (10-2000 μM); magnesium (Mg) and calcium (Ca) were tested at higher concentrations (200 and 50 mM, respectively). The influence on viability of human osteosarcoma cell line MG63, human umbilical cord perivascular (HUCPV) cells and mouse macrophages (RAW 264.7) was determined, as well as the induction of apoptosis and the expression of inflammatory factors (TNF-α, IL-1α). Significant differences between the applied cells could be observed. RAW exhibited the highest and HUCPV the lowest sensitivity. La and Ce showed the highest cytotoxicity of the analysed elements. Of the elements with high solubility in magnesium alloys, Gd and Dy seem to be more suitable than Y. The focus of magnesium alloy development for biomedical applications should include most defined alloy compositions with well-known tissue-specific and systemic effects.

KW - In vitro

KW - Inflammatory response

KW - Magnesium

KW - Rare earth elements

KW - Toxicity

KW - Engineering

UR - http://www.scopus.com/inward/record.url?scp=77954681094&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2009.09.024

DO - 10.1016/j.actbio.2009.09.024

M3 - Journal articles

C2 - 19800429

AN - SCOPUS:77954681094

VL - 6

SP - 1834

EP - 1842

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 5

ER -