Sustainable Production of the Cyanophycin Biopolymer in Tobacco in the Greenhouse and Field
Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet
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in: Frontiers in Bioengineering and Biotechnology, Jahrgang 10, 896863, 13.06.2022, S. 1-11.
Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet
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TY - JOUR
T1 - Sustainable Production of the Cyanophycin Biopolymer in Tobacco in the Greenhouse and Field
AU - Huckauf, Jana
AU - Brandt, Boudewijn P.
AU - Dezar, Carlos
AU - Nausch, Henrik
AU - Hauerwaas, Antoniya
AU - Weisenfeld, Ursula
AU - Elshiewy, Ossama
AU - Rua, Melina
AU - Hugenholtz, Jeroen
AU - Wesseler, Justus
AU - Cingiz, Kutay
AU - Broer, Inge
N1 - Conference code: 4
PY - 2022/6/13
Y1 - 2022/6/13
N2 - The production of biodegradable polymers as coproducts of other commercially relevant plant components can be a sustainable strategy to decrease the carbon footprint and increase the commercial value of a plant. The biodegradable polymer cyanophycin granular polypeptide (CGP) was expressed in the leaves of a commercial tobacco variety, whose seeds can serve as a source for biofuel and feed. In T0 generation in the greenhouse, up to 11% of the leaf dry weight corresponded to the CGP. In T1 generation, the maximum content decreased to approximately 4% dw, both in the greenhouse and first field trial. In the field, a maximum harvest of 4 g CGP/plant could be obtained. Independent of the CGP content, most transgenic plants exhibited a slight yield penalty in the leaf biomass, especially under stress conditions in greenhouse and field trials. After the harvest, the leaves were either Sun dried or ensiled. The resulting material was used to evaluate the extraction of CGP compared to that in the laboratory protocol. The farm-level analysis indicates that the extraction of CGP from tobacco plants can provide alternative income opportunities for tobacco farmers. The CGP yield/ha indicates that the CGP production in plants can be economically feasible depending on the cultivation and extraction costs. Moreover, we analyzed the consumer acceptance of potential applications associated with GM tobacco in four European countries (Germany, Finland, Italy and the Netherlands) and found unexpectedly high acceptance.
AB - The production of biodegradable polymers as coproducts of other commercially relevant plant components can be a sustainable strategy to decrease the carbon footprint and increase the commercial value of a plant. The biodegradable polymer cyanophycin granular polypeptide (CGP) was expressed in the leaves of a commercial tobacco variety, whose seeds can serve as a source for biofuel and feed. In T0 generation in the greenhouse, up to 11% of the leaf dry weight corresponded to the CGP. In T1 generation, the maximum content decreased to approximately 4% dw, both in the greenhouse and first field trial. In the field, a maximum harvest of 4 g CGP/plant could be obtained. Independent of the CGP content, most transgenic plants exhibited a slight yield penalty in the leaf biomass, especially under stress conditions in greenhouse and field trials. After the harvest, the leaves were either Sun dried or ensiled. The resulting material was used to evaluate the extraction of CGP compared to that in the laboratory protocol. The farm-level analysis indicates that the extraction of CGP from tobacco plants can provide alternative income opportunities for tobacco farmers. The CGP yield/ha indicates that the CGP production in plants can be economically feasible depending on the cultivation and extraction costs. Moreover, we analyzed the consumer acceptance of potential applications associated with GM tobacco in four European countries (Germany, Finland, Italy and the Netherlands) and found unexpectedly high acceptance.
KW - Management studies
KW - cost benefit analysis
KW - market analysis
KW - consumer acceptance
KW - lant made industrials
KW - sustainable production
KW - cost benefit analysis
KW - market analysis
KW - consumer acceptance
KW - Biology
KW - cyanophycin,
KW - field trial
KW - lant made industrials
KW - sustainable production
KW - cyanophycin
KW - plant made industrials
KW - sustainable production
KW - field trial
KW - isolation
UR - https://www.mendeley.com/catalogue/6497d50b-8005-32fb-89e1-45f53d4c2677/
UR - http://www.scopus.com/inward/record.url?scp=85133528253&partnerID=8YFLogxK
U2 - 10.3389/fbioe.2022.896863
DO - 10.3389/fbioe.2022.896863
M3 - Journal articles
C2 - 35769105
VL - 10
SP - 1
EP - 11
JO - Frontiers in Bioengineering and Biotechnology
JF - Frontiers in Bioengineering and Biotechnology
M1 - 896863
T2 - 4th Biennial Conference of the International Society for Plant Molecular Farming - ISPMF 2021
Y2 - 28 September 2021 through 29 September 2021
ER -