TY - JOUR

T1 - Production of fungal glucoamylase for glucose production from food waste

AU - Lam, Wan Chi

AU - Pleißner, Daniel

AU - Lin, Carol Sze Ki

PY - 2013/9/19

Y1 - 2013/9/19

N2 - The feasibility of using pastry waste as resource for glucoamylase (GA) production via solid state fermentation (SSF) was studied. The crude GA extract obtained was used for glucose production from mixed food waste. Our results showed that pastry waste could be used as a sole substrate for GA production. A maximal GA activity of 76.1 ± 6.1 U/mL was obtained at Day 10. The optimal pH and reaction temperature for the crude GA extract for hydrolysis were pH 5.5 and 55 °C, respectively. Under this condition, the half-life of the GA extract was 315.0 minutes with a deactivation constant (kd) 2.20 × 10−3 minutes−1. The application of the crude GA extract for mixed food waste hydrolysis and glucose production was successfully demonstrated. Approximately 53 g glucose was recovered from 100 g of mixed food waste in 1 h under the optimal digestion conditions, highlighting the potential of this approach as an alternative strategy for waste management and sustainable production of glucose applicable as carbon source in many biotechnological processes.

AB - The feasibility of using pastry waste as resource for glucoamylase (GA) production via solid state fermentation (SSF) was studied. The crude GA extract obtained was used for glucose production from mixed food waste. Our results showed that pastry waste could be used as a sole substrate for GA production. A maximal GA activity of 76.1 ± 6.1 U/mL was obtained at Day 10. The optimal pH and reaction temperature for the crude GA extract for hydrolysis were pH 5.5 and 55 °C, respectively. Under this condition, the half-life of the GA extract was 315.0 minutes with a deactivation constant (kd) 2.20 × 10−3 minutes−1. The application of the crude GA extract for mixed food waste hydrolysis and glucose production was successfully demonstrated. Approximately 53 g glucose was recovered from 100 g of mixed food waste in 1 h under the optimal digestion conditions, highlighting the potential of this approach as an alternative strategy for waste management and sustainable production of glucose applicable as carbon source in many biotechnological processes.

KW - Chemistry

KW - glucoamylase production

KW - Aspergillus awamori

KW - food waste hydrolysis

KW - glucose production

UR - http://www.scopus.com/inward/record.url?scp=85015598797&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/0932bc46-7ca4-3cc4-ac98-7952f8415b82/

U2 - 10.3390/biom3030651

DO - 10.3390/biom3030651

M3 - Journal articles

C2 - 24970186

VL - 3

SP - 651

EP - 661

JO - Biomolecules

JF - Biomolecules

SN - 2218-273X

IS - 3

ER -