TY - JOUR

T1 - Fermentative utilization of coffee mucilage using Bacillus coagulans and investigation of down-stream processing of fermentation broth for optically pure L(+)-lactic acid production

AU - Neu, Anna-Katrin

AU - Pleissner, Daniel

AU - Mehlmann, Kerstin

AU - Schneider, Roland

AU - Puerta-Quintero, Gloria Ines

AU - Venus, Joachim

PY - 2016/7/1

Y1 - 2016/7/1

N2 - In this study, mucilage, a residue from coffee production, was investigated as substrate in fermentative l(+)-lactic acid production. Mucilage was provided as liquid suspension consisting glucose, galactose, fructose, xylose and sucrose as free sugars (up to 60 g L−1), and used directly as medium in Bacillus coagulans batch fermentations carried out at 2 and 50 L scales. Using mucilage and 5 g L−1 yeast extract as additional nitrogen source, more than 40 g L−1 lactic acid was obtained. Productivity and yield were 4–5 g L−1 h−1 and 0.70–0.77 g lactic acid per g of free sugars, respectively, irrespective the scale. Similar yield was found when no yeast extract was supplied, the productivity, however, was 1.5 g L−1 h−1. Down-stream processing of culture broth, including filtration, electrodialysis, ion exchange chromatography and distillation, resulted in a pure lactic acid formulation containing 930 g L−1 l(+)-lactic acid. Optical purity was 99.8%.

AB - In this study, mucilage, a residue from coffee production, was investigated as substrate in fermentative l(+)-lactic acid production. Mucilage was provided as liquid suspension consisting glucose, galactose, fructose, xylose and sucrose as free sugars (up to 60 g L−1), and used directly as medium in Bacillus coagulans batch fermentations carried out at 2 and 50 L scales. Using mucilage and 5 g L−1 yeast extract as additional nitrogen source, more than 40 g L−1 lactic acid was obtained. Productivity and yield were 4–5 g L−1 h−1 and 0.70–0.77 g lactic acid per g of free sugars, respectively, irrespective the scale. Similar yield was found when no yeast extract was supplied, the productivity, however, was 1.5 g L−1 h−1. Down-stream processing of culture broth, including filtration, electrodialysis, ion exchange chromatography and distillation, resulted in a pure lactic acid formulation containing 930 g L−1 l(+)-lactic acid. Optical purity was 99.8%.

KW - Bacillus coagulans

KW - Nutrient recovery

KW - Renewable resources

KW - Coffee mucilage

KW - Waste utilization

KW - Chemistry

UR - http://www.scopus.com/inward/record.url?scp=84961675379&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2016.03.122

DO - 10.1016/j.biortech.2016.03.122

M3 - Journal articles

C2 - 27035470

VL - 211

SP - 398

EP - 405

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

IS - Juli

ER -