Fermentative utilization of coffee mucilage using Bacillus coagulans and investigation of down-stream processing of fermentation broth for optically pure L(+)-lactic acid production
Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet
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in: Bioresource Technology, Jahrgang 211, Nr. Juli, 01.07.2016, S. 398-405.
Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet
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TY - JOUR
T1 - Fermentative utilization of coffee mucilage using Bacillus coagulans and investigation of down-stream processing of fermentation broth for optically pure L(+)-lactic acid production
AU - Neu, Anna-Katrin
AU - Pleissner, Daniel
AU - Mehlmann, Kerstin
AU - Schneider, Roland
AU - Puerta-Quintero, Gloria Ines
AU - Venus, Joachim
PY - 2016/7/1
Y1 - 2016/7/1
N2 - In this study, mucilage, a residue from coffee production, was investigated as substrate in fermentative l(+)-lactic acid production. Mucilage was provided as liquid suspension consisting glucose, galactose, fructose, xylose and sucrose as free sugars (up to 60 g L−1), and used directly as medium in Bacillus coagulans batch fermentations carried out at 2 and 50 L scales. Using mucilage and 5 g L−1 yeast extract as additional nitrogen source, more than 40 g L−1 lactic acid was obtained. Productivity and yield were 4–5 g L−1 h−1 and 0.70–0.77 g lactic acid per g of free sugars, respectively, irrespective the scale. Similar yield was found when no yeast extract was supplied, the productivity, however, was 1.5 g L−1 h−1. Down-stream processing of culture broth, including filtration, electrodialysis, ion exchange chromatography and distillation, resulted in a pure lactic acid formulation containing 930 g L−1 l(+)-lactic acid. Optical purity was 99.8%.
AB - In this study, mucilage, a residue from coffee production, was investigated as substrate in fermentative l(+)-lactic acid production. Mucilage was provided as liquid suspension consisting glucose, galactose, fructose, xylose and sucrose as free sugars (up to 60 g L−1), and used directly as medium in Bacillus coagulans batch fermentations carried out at 2 and 50 L scales. Using mucilage and 5 g L−1 yeast extract as additional nitrogen source, more than 40 g L−1 lactic acid was obtained. Productivity and yield were 4–5 g L−1 h−1 and 0.70–0.77 g lactic acid per g of free sugars, respectively, irrespective the scale. Similar yield was found when no yeast extract was supplied, the productivity, however, was 1.5 g L−1 h−1. Down-stream processing of culture broth, including filtration, electrodialysis, ion exchange chromatography and distillation, resulted in a pure lactic acid formulation containing 930 g L−1 l(+)-lactic acid. Optical purity was 99.8%.
KW - Bacillus coagulans
KW - Nutrient recovery
KW - Renewable resources
KW - Coffee mucilage
KW - Waste utilization
KW - Chemistry
UR - http://www.scopus.com/inward/record.url?scp=84961675379&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2016.03.122
DO - 10.1016/j.biortech.2016.03.122
M3 - Journal articles
C2 - 27035470
VL - 211
SP - 398
EP - 405
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
IS - Juli
ER -