Heterotrophic growth of Galdieria sulphuraria on residues from aquaculture and fish processing industries

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  • Daniel Pleissner
  • Stephanie Schönfelder
  • Nicole Händel
  • Julia Dalichow
  • Judith Ettinger
  • Kristine Kvangarsnes
  • Egidijus Dauksas
  • Turid Rustad
  • Janna Cropotova

The study aimed at zero-waste utilization of fish processing streams for cultivation of microalgae Galdieria sulphuraria. Wastewater from a fish processing facility, slam (mix of used fish feed and faeces), and dried pellet (sediments after enzymatic hydrolysis of rainbow trout) were investigated as potential sources of carbon, nitrogen, and phosphate for cultivation of G. sulphuraria. The pellet extract was found to support the growth of G. sulphuraria when appropriate diluted, at concentrations below 40 % (v/v). It was revealed that wastewater does not impact the growth negatively, however free amino nitrogen and carbon sources need to be supplied from another source. Therefore, only proteolyzed pellet extract (20 %, v/v) was selected for upscaling and a biomass concentration of 80 g L−1 (growth rate was 0.72 day−1) was achieved in a non-sterile fed-batch culture. Even though biomass was produced under non-sterile conditions no pathogens such as Salmonella sp. could be detected.

Original languageEnglish
Article number129281
JournalBioresource Technology
Number of pages8
Publication statusPublished - 01.09.2023

Bibliographical note

This work was financially supported by the German Federal Ministry of Food and Agriculture /BMEL) through the Federal Office for Agriculture and Food /BLE), grant number 2821ERA12C. This project has received funding from the European Union’s Horizon 2020 research and innovation program under the grant agreement No 862555. Janna Cropotova acknowledges the FOSC-ERA project “CLIMAQUA”, (grant No. 327695 of BIONÆR-Bionæringsprogram) for support.

Publisher Copyright:
© 2023 The Author(s)

    Research areas

  • Algal biomass, Bioeconomy, Bioprocess, Fish processing, Protein residues
  • Biology