TY - JOUR

T1 - Rhizosphere microbiome modulated effects of biochar on ryegrass 15N uptake and rhizodeposited 13C allocation in soil

AU - Fu, Yingyi

AU - Kumar, Amit

AU - Chen, Lijun

AU - Jiang, Yuji

AU - Ling, Ning

AU - Wang, Runze

AU - Pan, Qiong

AU - Singh, Bhupinder Pal

AU - Redmile-Gordon, Marc

AU - Luan, Lu

AU - Li, Qin

AU - Shi, Quan

AU - Reid, Brian J.

AU - Fang, Yunying

AU - Kuzyakov, Yakov

AU - Luo, Yu

AU - Xu, Jianming

N1 - This study was supported by the National Science Foundation of China (41520104001; 41671233), and Fundamental Research Funds for the Central Universities (2019QNA6012).

PY - 2021/6

Y1 - 2021/6

N2 - Background and aims: Incorporation of biochar into the soil sequesters C for millennia, but the concomitant effects on plant rhizodepositions and nutrient (e.g., nitrogen; N) trade-offs via interactions of heterotrophic microbiota, might offset this sequestration. Methods: Ryegrass (Lolium perenne L.) with and without biochar amendment were pulse labelled in a 13CO2 atmosphere and 15N fertilizer added. Ryegrass and soils were destructively sampled at 16 and 30 days after seedling emergence. Isotope analysis was coupled with MiSeq sequencing of bacterial (16s rRNA) and fungal (ITS) genes to identify the effect of biochar on the associated microbiota involved in 13C allocation into soil aggregates and promotion of 15N uptake by L. perenne. Results: Biochar increased root biomass and 15N uptake but decreased rhizodeposited-13C recovery from large and small macroaggregates (by 12–57% and 57–72%, respectively). These changes in 13C flow and 15N uptake were accompanied by an increase in microbial biomass, and enhanced negative correlations between bacteria and fungi. O2PLS indicated members of seventeen genera that were correlated with soil stabilization of rhizodeposits in soil and plant N-uptake. For instance, Xanthomonadales (Proteobacteria) and RB41 (Acidobacteria), previously reported to be plant growth promoting rhizobacteria, were found to be positively correlated with 15N uptake by L. perenne. Conclusions: Our research explored the genera associated with biochar-modified 15N uptake by Lolium perenne and photosynthate 13C allocation into soil aggregates. Future research with SIP is required to fully assess microbial turnover, the ubiquity of similar rhizosphere microbiota and their fundamental importance for sequestration in the plant-soil-microbe-biochar systems.

AB - Background and aims: Incorporation of biochar into the soil sequesters C for millennia, but the concomitant effects on plant rhizodepositions and nutrient (e.g., nitrogen; N) trade-offs via interactions of heterotrophic microbiota, might offset this sequestration. Methods: Ryegrass (Lolium perenne L.) with and without biochar amendment were pulse labelled in a 13CO2 atmosphere and 15N fertilizer added. Ryegrass and soils were destructively sampled at 16 and 30 days after seedling emergence. Isotope analysis was coupled with MiSeq sequencing of bacterial (16s rRNA) and fungal (ITS) genes to identify the effect of biochar on the associated microbiota involved in 13C allocation into soil aggregates and promotion of 15N uptake by L. perenne. Results: Biochar increased root biomass and 15N uptake but decreased rhizodeposited-13C recovery from large and small macroaggregates (by 12–57% and 57–72%, respectively). These changes in 13C flow and 15N uptake were accompanied by an increase in microbial biomass, and enhanced negative correlations between bacteria and fungi. O2PLS indicated members of seventeen genera that were correlated with soil stabilization of rhizodeposits in soil and plant N-uptake. For instance, Xanthomonadales (Proteobacteria) and RB41 (Acidobacteria), previously reported to be plant growth promoting rhizobacteria, were found to be positively correlated with 15N uptake by L. perenne. Conclusions: Our research explored the genera associated with biochar-modified 15N uptake by Lolium perenne and photosynthate 13C allocation into soil aggregates. Future research with SIP is required to fully assess microbial turnover, the ubiquity of similar rhizosphere microbiota and their fundamental importance for sequestration in the plant-soil-microbe-biochar systems.

KW - C pulse labelling

KW - N fertilizers

KW - Aggregates compositions; rhizosphere microbiome

KW - Biochar functions

KW - Carbon sequestration

KW - Rhizodeposits

KW - Ecosystems Research

UR - http://www.scopus.com/inward/record.url?scp=85103180595&partnerID=8YFLogxK

U2 - 10.1007/s11104-021-04845-9

DO - 10.1007/s11104-021-04845-9

M3 - Journal articles

AN - SCOPUS:85103180595

VL - 463

SP - 359

EP - 377

JO - Plant and Soil

JF - Plant and Soil

SN - 0032-079X

IS - 1-2

ER -