Rhizosphere microbiome modulated effects of biochar on ryegrass 15N uptake and rhizodeposited 13C allocation in soil
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In: Plant and Soil, Vol. 463, No. 1-2, 06.2021, p. 359-377.
Research output: Journal contributions › Journal articles › Research › peer-review
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TY - JOUR
T1 - Rhizosphere microbiome modulated effects of biochar on ryegrass 15N uptake and rhizodeposited 13C allocation in soil
AU - Fu, Yingyi
AU - Kumar, Amit
AU - Chen, Lijun
AU - Jiang, Yuji
AU - Ling, Ning
AU - Wang, Runze
AU - Pan, Qiong
AU - Singh, Bhupinder Pal
AU - Redmile-Gordon, Marc
AU - Luan, Lu
AU - Li, Qin
AU - Shi, Quan
AU - Reid, Brian J.
AU - Fang, Yunying
AU - Kuzyakov, Yakov
AU - Luo, Yu
AU - Xu, Jianming
PY - 2021/6
Y1 - 2021/6
N2 - Background and aims: Incorporation of biochar into the soil sequesters C for millennia, but the concomitant effects on plant rhizodepositions and nutrient (e.g., nitrogen; N) trade-offs via interactions of heterotrophic microbiota, might offset this sequestration. Methods: Ryegrass (Lolium perenne L.) with and without biochar amendment were pulse labelled in a 13CO2 atmosphere and 15N fertilizer added. Ryegrass and soils were destructively sampled at 16 and 30 days after seedling emergence. Isotope analysis was coupled with MiSeq sequencing of bacterial (16s rRNA) and fungal (ITS) genes to identify the effect of biochar on the associated microbiota involved in 13C allocation into soil aggregates and promotion of 15N uptake by L. perenne. Results: Biochar increased root biomass and 15N uptake but decreased rhizodeposited-13C recovery from large and small macroaggregates (by 12–57% and 57–72%, respectively). These changes in 13C flow and 15N uptake were accompanied by an increase in microbial biomass, and enhanced negative correlations between bacteria and fungi. O2PLS indicated members of seventeen genera that were correlated with soil stabilization of rhizodeposits in soil and plant N-uptake. For instance, Xanthomonadales (Proteobacteria) and RB41 (Acidobacteria), previously reported to be plant growth promoting rhizobacteria, were found to be positively correlated with 15N uptake by L. perenne. Conclusions: Our research explored the genera associated with biochar-modified 15N uptake by Lolium perenne and photosynthate 13C allocation into soil aggregates. Future research with SIP is required to fully assess microbial turnover, the ubiquity of similar rhizosphere microbiota and their fundamental importance for sequestration in the plant-soil-microbe-biochar systems.
AB - Background and aims: Incorporation of biochar into the soil sequesters C for millennia, but the concomitant effects on plant rhizodepositions and nutrient (e.g., nitrogen; N) trade-offs via interactions of heterotrophic microbiota, might offset this sequestration. Methods: Ryegrass (Lolium perenne L.) with and without biochar amendment were pulse labelled in a 13CO2 atmosphere and 15N fertilizer added. Ryegrass and soils were destructively sampled at 16 and 30 days after seedling emergence. Isotope analysis was coupled with MiSeq sequencing of bacterial (16s rRNA) and fungal (ITS) genes to identify the effect of biochar on the associated microbiota involved in 13C allocation into soil aggregates and promotion of 15N uptake by L. perenne. Results: Biochar increased root biomass and 15N uptake but decreased rhizodeposited-13C recovery from large and small macroaggregates (by 12–57% and 57–72%, respectively). These changes in 13C flow and 15N uptake were accompanied by an increase in microbial biomass, and enhanced negative correlations between bacteria and fungi. O2PLS indicated members of seventeen genera that were correlated with soil stabilization of rhizodeposits in soil and plant N-uptake. For instance, Xanthomonadales (Proteobacteria) and RB41 (Acidobacteria), previously reported to be plant growth promoting rhizobacteria, were found to be positively correlated with 15N uptake by L. perenne. Conclusions: Our research explored the genera associated with biochar-modified 15N uptake by Lolium perenne and photosynthate 13C allocation into soil aggregates. Future research with SIP is required to fully assess microbial turnover, the ubiquity of similar rhizosphere microbiota and their fundamental importance for sequestration in the plant-soil-microbe-biochar systems.
KW - C pulse labelling
KW - N fertilizers
KW - Aggregates compositions; rhizosphere microbiome
KW - Biochar functions
KW - Carbon sequestration
KW - Rhizodeposits
KW - Ecosystems Research
UR - http://www.scopus.com/inward/record.url?scp=85103180595&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/6241d75e-c120-365a-aa38-816e9d404213/
U2 - 10.1007/s11104-021-04845-9
DO - 10.1007/s11104-021-04845-9
M3 - Journal articles
AN - SCOPUS:85103180595
VL - 463
SP - 359
EP - 377
JO - Plant and Soil
JF - Plant and Soil
SN - 0032-079X
IS - 1-2
ER -