A comprehensive method for determination of fatty acids in the initial oral biofilm (pellicle)

Research output: Journal contributionsJournal articlesResearchpeer-review

Authors

The acquired pellicle is a tenacious organic layer covering the surface of teeth, protecting the underlying dental hard tissues. Lipids account for about one quarter of the pellicle's dry weight and are assumed to be of considerable importance for their protective properties. Nevertheless, only preliminary information is available about the nature of lipids in the pellicle. Gas chromatography coupled with electron impact ionization mass spectrometry was used to establish a convenient analytical protocol in order to obtain a qualitative and quantitative characterization of a wide range of FAs (C 12-C 22). In situ biofilm formation was performed on bovine enamel slabs mounted on individual splints carried by 10 subjects. A modified Folch extraction procedure was adopted to extract the lipids from the detached pellicle, followed by transesterification to fatty acid methyl esters using methanol and concentrated hydrochloric acid. Tridecanoic and nonadecanoic acid were used as internal standards suitable and reliable for robust, precise and accurate measurements. The present study demonstrates, for the first time, a procedure based on a combination of innovative specimen generation and convenient sample preparation with sensitive GC-MS analysis for the determination of the fatty acid profile of the initial oral biofilm.

Original languageEnglish
JournalJournal of Lipid Research
Volume53
Issue number10
Pages (from-to)2226-2230
Number of pages5
DOIs
Publication statusPublished - 01.10.2012

DOI

Recently viewed

Publications

  1. National ecosystem restoration pledges are mismatched with social-ecological enabling conditions
  2. A comparative survey of chemistry-driven in silico methods to identify hazardous substances under REACH
  3. Photodegradation of the UV filter ethylhexyl methoxycinnamate under ultraviolet light
  4. Group evaluations as self-group distancing
  5. Understanding the role of gender identity in charitable giving—recruiting bone marrow donors
  6. D2R2 2024: Linked Data-driven Resilience Research 2024
  7. Work-in-Progress
  8. Regierung
  9. Pragmatics and the English Language, Jonathan Culpeper, Michael Haugh. Palgrave Macmillan, Basingstoke (2014), 316 pp., ISBN: 9780230551732
  10. Ex Machina
  11. So verknüpfen Sie operatives Controlling und Berichtswesen
  12. The perceiver’s social role and a risk’s causal structure as determinants of environmental risk evaluation
  13. Non-native Douglas fir promotes epigeal spider density, but has a mixed effect on functional diversity
  14. Determinants of pollution
  15. Mindfulness and cognitive-behavioral strategies for psychological detachment
  16. Global decoupling of functional and phylogenetic diversity in plant communities
  17. COVID-19 and the ageing workforce
  18. Manieren beibringen
  19. A stakeholder theory perspective on business models: Value creation for sustainability
  20. Joint proceedings of RSP 2017 and QuWeDa 2017
  21. Regulation of a servo piezo mechanical hydraulic actuator for intake valves in camless combustion engines
  22. Is ‘waste’ an appropriate concept in a sustainable bioeconomy?
  23. Social and Ecological Elements for a Perspective Approach to Citizen Science on the Beach