The aim of our research is the development of a prototype of a Bionanosensor, which can detected the pathogens (virus, bacteria) in enclosed spaces (such as hospitals) in real time. The sensor based on Polymers nanofibers, which act as sensing element. Therefore is important to investigate the geometrical, mechanical and electrical properties of it, in order to understand better its properties for the sensor application. The sensitivity of the DC electrical properties of various nanowires to molecules adsorbed on their surfaces [8] also raises the question of whether similar AC conductance changes exist and can be exploited for Biosensor purposes. The nanofiber is produced by electrospinning method, which is a cost-effective and versatile process to fabricate nanofibers from a wide range of materials at room temperature and atmospheric pressure, of which the diameter ranges from tens of nanometers to a few micrometers, see Fig. 2. The research involved experimental and theoretical efforts to investigate and understand high frequency (MHz through tens of GHz) electrodynamic response properties of nanowires of polymer and conductive polymer. We have analysed the electronic properties (the impedance) of the electrospun using a coplanar μ-strip (CPμS) sensor, where the electrospun is the fibers net resulting from the electrospinning process. Here we present a model for the sensor based on the transmission line theory, see Fig. 5. The electrospun impedance is modeled by a conductance and capacitance in parallel with the transmission line sensor's model. The model was simulated with MATLAB and its result was compared with the experimental data of the CPμS in order to see if the model is agreement with the experiment, so we can see how good the model of the sensor is. After the validation of the sensor prototype we have measured the impedance of the electrospun using the CPμS. First we measure the impedance characteristic of the sensor without electrospun and after that the electrospun is deposited by electrospinning on the surface of the CPμS sensor, the impedance characteristic of the system CPμS/ Electrospun was measured. From the plotted of the experimental data and we have obtained two curves, one for the sensor without electrospun and one for the CPμS/ Electrospun system, see Fig. 4. From the difference between both curves we can see that is possible to measure the impedance, conductance and the capacitance of the polymer electrospun. We have found that the the complex AC conductance of the polymer electrospun exhibited a sub-linear power law decrease with frequency that is consistent with behavior found in polymer (insulator).