TY - JOUR

T1 - Fermentative lactic acid production from coffee pulp hydrolysate using Bacillus coagulans at laboratory and pilot scales

AU - Pleissner, Daniel

AU - Neu, Anna-Katrin

AU - Mehlmann, Kerstin

AU - Schneider, Roland

AU - Puerta-Quintero, Gloria Inés

AU - Venus, Joachim

PY - 2016/10/1

Y1 - 2016/10/1

N2 - In this study, the lignocellulosic residue coffee pulp was used as carbon source in fermentative l(+)-lactic acid production using Bacillus coagulans. After thermo-chemical treatment at 121 °C for 30 min in presence of 0.18 mol L−1 H2SO4 and following an enzymatic digestion using Accellerase 1500 carbon-rich hydrolysates were obtained. Two different coffee pulp materials with comparable biomass composition were used, but sugar concentrations in hydrolysates showed variations. The primary sugars were (g L−1) glucose (20–30), xylose (15–25), sucrose (5–11) and arabinose (0.7–10). Fermentations were carried out at laboratory (2 L) and pilot (50 L) scales in presence of 10 g L−1 yeast extract. At pilot scale carbon utilization and lactic acid yield per gram of sugar consumed were 94.65% and 0.78 g g−1, respectively. The productivity was 4.02 g L−1 h−1. Downstream processing resulted in a pure formulation containing 937 g L−1 l(+)-lactic acid with an optical purity of 99.7%.

AB - In this study, the lignocellulosic residue coffee pulp was used as carbon source in fermentative l(+)-lactic acid production using Bacillus coagulans. After thermo-chemical treatment at 121 °C for 30 min in presence of 0.18 mol L−1 H2SO4 and following an enzymatic digestion using Accellerase 1500 carbon-rich hydrolysates were obtained. Two different coffee pulp materials with comparable biomass composition were used, but sugar concentrations in hydrolysates showed variations. The primary sugars were (g L−1) glucose (20–30), xylose (15–25), sucrose (5–11) and arabinose (0.7–10). Fermentations were carried out at laboratory (2 L) and pilot (50 L) scales in presence of 10 g L−1 yeast extract. At pilot scale carbon utilization and lactic acid yield per gram of sugar consumed were 94.65% and 0.78 g g−1, respectively. The productivity was 4.02 g L−1 h−1. Downstream processing resulted in a pure formulation containing 937 g L−1 l(+)-lactic acid with an optical purity of 99.7%.

KW - Bacillus coagulans

KW - Renewable resources

KW - Coffee pulp

KW - Agricultural residue utilization

KW - Chemistry

UR - http://www.scopus.com/inward/record.url?scp=84975796045&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2016.06.078

DO - 10.1016/j.biortech.2016.06.078

M3 - Journal articles

C2 - 27359065

VL - 218

SP - 167

EP - 173

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -