Fermentative lactic acid production from coffee pulp hydrolysate using Bacillus coagulans at laboratory and pilot scales
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In: Bioresource Technology, Vol. 218, 01.10.2016, p. 167-173.
Research output: Journal contributions › Journal articles › Research › peer-review
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T1 - Fermentative lactic acid production from coffee pulp hydrolysate using Bacillus coagulans at laboratory and pilot scales
AU - Pleissner, Daniel
AU - Neu, Anna-Katrin
AU - Mehlmann, Kerstin
AU - Schneider, Roland
AU - Puerta-Quintero, Gloria Inés
AU - Venus, Joachim
PY - 2016/10/1
Y1 - 2016/10/1
N2 - In this study, the lignocellulosic residue coffee pulp was used as carbon source in fermentative l(+)-lactic acid production using Bacillus coagulans. After thermo-chemical treatment at 121 °C for 30 min in presence of 0.18 mol L−1 H2SO4 and following an enzymatic digestion using Accellerase 1500 carbon-rich hydrolysates were obtained. Two different coffee pulp materials with comparable biomass composition were used, but sugar concentrations in hydrolysates showed variations. The primary sugars were (g L−1) glucose (20–30), xylose (15–25), sucrose (5–11) and arabinose (0.7–10). Fermentations were carried out at laboratory (2 L) and pilot (50 L) scales in presence of 10 g L−1 yeast extract. At pilot scale carbon utilization and lactic acid yield per gram of sugar consumed were 94.65% and 0.78 g g−1, respectively. The productivity was 4.02 g L−1 h−1. Downstream processing resulted in a pure formulation containing 937 g L−1 l(+)-lactic acid with an optical purity of 99.7%.
AB - In this study, the lignocellulosic residue coffee pulp was used as carbon source in fermentative l(+)-lactic acid production using Bacillus coagulans. After thermo-chemical treatment at 121 °C for 30 min in presence of 0.18 mol L−1 H2SO4 and following an enzymatic digestion using Accellerase 1500 carbon-rich hydrolysates were obtained. Two different coffee pulp materials with comparable biomass composition were used, but sugar concentrations in hydrolysates showed variations. The primary sugars were (g L−1) glucose (20–30), xylose (15–25), sucrose (5–11) and arabinose (0.7–10). Fermentations were carried out at laboratory (2 L) and pilot (50 L) scales in presence of 10 g L−1 yeast extract. At pilot scale carbon utilization and lactic acid yield per gram of sugar consumed were 94.65% and 0.78 g g−1, respectively. The productivity was 4.02 g L−1 h−1. Downstream processing resulted in a pure formulation containing 937 g L−1 l(+)-lactic acid with an optical purity of 99.7%.
KW - Bacillus coagulans
KW - Renewable resources
KW - Coffee pulp
KW - Agricultural residue utilization
KW - Chemistry
UR - http://www.scopus.com/inward/record.url?scp=84975796045&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2016.06.078
DO - 10.1016/j.biortech.2016.06.078
M3 - Journal articles
C2 - 27359065
VL - 218
SP - 167
EP - 173
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -