The aim of this study was to perform a Life Cycle Assessment of a production process of 1 kg dry algal biomass powder (Galdieria sulphuraria) with 27 % (w/w) protein content for human consumption for optimizing the production regarding global warming potential and resource efficiency in combination with food waste utilization. It was investigated, underpinned by a comparison of the use of conventional glucose, whether and to what extent the environmental impact/global warming potential can be reduced by changing to food waste hydrolysate and how this can lead to a more sustainable use of resources and a sustainable development. Overall, the results showed that hydrolysis, along with freeze-drying, caused most of the overall impact. The carbon footprint associated with the use of hydrolyzed food waste was 11% higher than using conventional glucose and supplementary nutrients mainly driven by the high demand of energy for hydrolysis.