We conducted a systematic review of the available peer-reviewed literature that specifically focuses on the combination of sustainability and gender. We analyzed the existing peer-reviewed research regarding the extent to which gender plays a role in the empirical literature, how this is methodologically collected and what understanding of gender is applied in those articles. Our aim is to provide an overview of the current most common fields of research and thus show in which areas gender is already being included in the sustainability sciences and to what extent and in which areas this inclusion has not yet taken place or has only taken place to a limited extent. We identified 1054 papers that matched our criteria and conducted research on at least one sustainable development goal and gender research. Within these papers (i), the overall number of countries where lead authors were located was very high (91 countries). While the majority of lead authors were located in the Global North, less than a third of the articles were led by authors located in the Global South. Furthermore, gender is often just used as a category of empirical analysis rather than a research focus. We were able to identify (ii) a lack in coherent framing of relevant terms. Often no definition of sustainability was given, and only the sustainability goals (SDGs or MDGs) were used as a framework to refer to sustainability. Both gender and sustainability were often used as key words without being specifically addressed. Concerning the knowledge types of sustainability, our expectation that system knowledge dominates the literature was confirmed. While a problem orientation dominates much of the discourse, only a few papers focus on normative or transformative knowledge. (iii) Furthermore, the investigated literature was mainly contributing to few SDGs, with SDG 5 ‘Gender Equality’ accounting for 83% of all contributions, followed by SDG 8 ‘Decent Work and Economic Growth’ (21%), SDG 3 ‘Good Health and Well-being’ (15%) and SDG 4 ‘Quality Education’ (12%). We were additionally able to identify seven research clusters in the landscape of gender in sustainability science. (iv) A broad range of diverse methods was utilized that allow us to approximate different forms of knowledge. Yet within different research clusters, the spectrum of methodologies is rather homogeneous. (v) Overall, in most papers gender is conceptualized in binary terms. In most cases, the research is explicitly about women, running the risk that gender research in sustainability sciences grows into a synonym for women's studies.