The use of a monolithic column to improve the simultaneous determination of caffeine, paracetamol, pseudoephedrine, aspirin, dextromethorphan, chlorpheniramine in pharmaceutical formulations by HPLC-A comparison with a conventional reversed-phase silica-based column

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Authors

  • Ghada M. Hadad
  • Waleed M. M. Mahmoud
A new simple, rapid, and sensitive liquid chromatographic method has been developed and validated for the simultaneous determination of caffeine, paracetamol, pseudoephedrine, aspirin, dextromethorphan, and chlorpheniramine in pharmaceutical tablet formulations and was performed on conventional C 18 and monolithic silica C 18 columns. Chromatographic separation was achieved by using a mixture of methanol and a buffer containing 10mM NaH 2PO 4, 3mM heptane sulfonic acid sodium salt, 0.1% triethylamine, pH 2.5 (adjusted with hydrochloric acid) in a ratio 50:50v/v, 40:60v/v for conventional C 18 and monolithic silica C 18 columns, respectively. Quantitation was achieved with UV detection at 214nm, based on peak area. The proposed method could be used in the presence of salicylic acid as impurity or decomposition product in dosage forms containing aspirin. The proposed method was validated and successfully applied for the analysis of pharmaceutical formulations and laboratory-prepared mixtures containing these multicomponent combinations.
Translated title of the contributionDer Gebrauch einer monolithischen Säule zur verbesserten simultanen Bestimmung von Koffein, Paracetamol, Pseudoephedrin, Aspirin, Dextromethorphan und Chlorphentramin in pharmazeutischen Formulierungen mittels HPLC - Vergleich mit einer konmventionellen RP-Säule auf Silikabasis
Original languageEnglish
JournalJournal of Liquid Chromatography and Related Technologies
Volume34
Issue number20
Pages (from-to)2516–2532
Number of pages17
ISSN1082-6076
DOIs
Publication statusPublished - 01.12.2011

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