The use of a monolithic column to improve the simultaneous determination of caffeine, paracetamol, pseudoephedrine, aspirin, dextromethorphan, chlorpheniramine in pharmaceutical formulations by HPLC-A comparison with a conventional reversed-phase silica-based column
Research output: Journal contributions › Journal articles › Research › peer-review
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In: Journal of Liquid Chromatography and Related Technologies, Vol. 34, No. 20, 01.12.2011, p. 2516–2532.
Research output: Journal contributions › Journal articles › Research › peer-review
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TY - JOUR
T1 - The use of a monolithic column to improve the simultaneous determination of caffeine, paracetamol, pseudoephedrine, aspirin, dextromethorphan, chlorpheniramine in pharmaceutical formulations by HPLC-A comparison with a conventional reversed-phase silica-based column
AU - Hadad, Ghada M.
AU - Mahmoud, Waleed M. M.
PY - 2011/12/1
Y1 - 2011/12/1
N2 - A new simple, rapid, and sensitive liquid chromatographic method has been developed and validated for the simultaneous determination of caffeine, paracetamol, pseudoephedrine, aspirin, dextromethorphan, and chlorpheniramine in pharmaceutical tablet formulations and was performed on conventional C 18 and monolithic silica C 18 columns. Chromatographic separation was achieved by using a mixture of methanol and a buffer containing 10mM NaH 2PO 4, 3mM heptane sulfonic acid sodium salt, 0.1% triethylamine, pH 2.5 (adjusted with hydrochloric acid) in a ratio 50:50v/v, 40:60v/v for conventional C 18 and monolithic silica C 18 columns, respectively. Quantitation was achieved with UV detection at 214nm, based on peak area. The proposed method could be used in the presence of salicylic acid as impurity or decomposition product in dosage forms containing aspirin. The proposed method was validated and successfully applied for the analysis of pharmaceutical formulations and laboratory-prepared mixtures containing these multicomponent combinations.
AB - A new simple, rapid, and sensitive liquid chromatographic method has been developed and validated for the simultaneous determination of caffeine, paracetamol, pseudoephedrine, aspirin, dextromethorphan, and chlorpheniramine in pharmaceutical tablet formulations and was performed on conventional C 18 and monolithic silica C 18 columns. Chromatographic separation was achieved by using a mixture of methanol and a buffer containing 10mM NaH 2PO 4, 3mM heptane sulfonic acid sodium salt, 0.1% triethylamine, pH 2.5 (adjusted with hydrochloric acid) in a ratio 50:50v/v, 40:60v/v for conventional C 18 and monolithic silica C 18 columns, respectively. Quantitation was achieved with UV detection at 214nm, based on peak area. The proposed method could be used in the presence of salicylic acid as impurity or decomposition product in dosage forms containing aspirin. The proposed method was validated and successfully applied for the analysis of pharmaceutical formulations and laboratory-prepared mixtures containing these multicomponent combinations.
KW - Chemistry
KW - chromatography
KW - pharmaceuticals
KW - COLUMN
KW - monolithic
KW - aspirin
KW - caffeine
KW - chlorpheniramine
KW - dextromethorphan
KW - paracetamol
KW - pseudoephedrine
KW - Sustainability Science
UR - http://www.scopus.com/inward/record.url?scp=84856257198&partnerID=8YFLogxK
U2 - 10.1080/10826076.2011.591031
DO - 10.1080/10826076.2011.591031
M3 - Journal articles
VL - 34
SP - 2516
EP - 2532
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
SN - 1082-6076
IS - 20
ER -