The present study is a validation study asking the question if the method of using thinking-aloud protocols (TAPs) for investigating learning effects is appropriate, as the process of thinking aloud could play a crucial role in cognitive processing by prompting self-regulative processes and could therefore interfere with learning. The present work uses the negative learning effect of seductive details to investigate this research question and is thereby also offering a new perspective from research on self-regulation on the discussion of the seductive details effect. 120 university students learned with a digital learning program that was varied by the two factors thinking-aloud (with vs. without) and seductive details (with vs. without) in the 2x2 factorial design study. Results show that TAPs affect visual information processing measured by eye movement, subjectively perceived cognitive load, and retention performance, but not comprehension or transfer performance. Moreover, the seductive details effect is confirmed to be stable also under the TAPs condition, as no interaction effects were found. These findings have several interesting theoretical and practical implications that are discussed from three viewpoints, the methodological, the research on seductive details and the self-regulation perspective.