Sustainable Single-Stage Solid-Liquid Extraction of Hesperidin and Rutin from Agro-Products Using Cyrene

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschungbegutachtet


  • Roxana Alina Milescu
  • Mateus Lodi Segatto
  • Aylon Stahl
  • Con Robert Mcelroy
  • Thomas James Farmer
  • James Hanley Clark
  • Vânia Gomes Zuin

Hesperidin and rutin are two sought-after natural flavonoids, traditionally extracted from abundant natural citrus fruits and tea leaves using large amounts of ethanol or methanol solvents. Recent trends in extractions have focused on minimizing the use of solvents and creating simpler cost-effective processes. This study aims to apply the concept of chemical valorization in the context of a circular economy, by using agro-industrial waste and biobased alternatives to traditional solvents, which are of environmental concern. We use minimum amounts of solvent/sample (5 mL/0.25 or 0.5 g) to extract hesperidin and rutin in a single-stage solid-liquid extraction. Thirty individual solvents and HSPiP were applied to find the best solvents/blends for extraction. The type of solvent, sample preparation, maceration time, and extraction temperature were studied. Results showed that the biobased solvent Cyrene is very effective when mildly heated to 65 °C (up to 91%) or mixed with water. Adding water to Cyrene forms its geminal diol hydrate, this enhances the solubility and extraction of hesperidin and rutin up to ten times than those of the original pure ketone form. Quantitative sustainability metrics from the CHEM21 Toolkit demonstrated that our extraction methodology is environmentally friendly and offers future potential of isolation of other flavonoids.

ZeitschriftACS Sustainable Chemistry and Engineering
Seiten (von - bis)18245-18257
Anzahl der Seiten13
PublikationsstatusErschienen - 14.12.2020

Bibliographische Notiz

Funding Information:
We thank Circa Group and RenewChem for funding this research work. This study was part-funded by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil (CAPES) - Finance Code 001, CNPq and FAPESP (process numbers: 14/50827-1; 2017/25015-1; 2018/11409-,0).

Publisher Copyright:
© 2020 American Chemical Society.