Kinetic and Stoichiometric Modeling-Based Analysis of Docosahexaenoic Acid (DHA) Production Potential by Crypthecodinium cohnii from Glycerol, Glucose and Ethanol

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschungbegutachtet

Authors

  • Kristaps Berzins
  • Reinis Muiznieks
  • Matiss R. Baumanis
  • Inese Strazdina
  • Karlis Shvirksts
  • Santa Prikule
  • Vytautas Galvanauskas
  • Daniel Pleissner
  • Agris Pentjuss
  • Mara Grube
  • Uldis Kalnenieks
  • Egils Stalidzans

Docosahexaenoic acid (DHA) is one of the most important long-chain polyunsaturated fatty acids (LC-PUFAs), with numerous health benefits. Crypthecodinium cohnii, a marine hetero-trophic dinoflagellate, is successfully used for the industrial production of DHA because it can ac-cumulate DHA at high concentrations within the cells. Glycerol is an interesting renewable substrate for DHA production since it is a by-product of biodiesel production and other industries, and is globally generated in large quantities. The DHA production potential from glycerol, ethanol and glucose is compared by combining fermentation experiments with the pathway-scale kinetic modeling and constraint-based stoichiometric modeling of C. cohnii metabolism. Glycerol has the slow-est biomass growth rate among the tested substrates. This is partially compensated by the highest PUFAs fraction, where DHA is dominant. Mathematical modeling reveals that glycerol has the best experimentally observed carbon transformation rate into biomass, reaching the closest values to the theoretical upper limit. In addition to our observations, the published experimental evidence indi-cates that crude glycerol is readily consumed by C. cohnii, making glycerol an attractive substrate for DHA production.

OriginalspracheEnglisch
Aufsatznummer115
ZeitschriftMarine Drugs
Jahrgang20
Ausgabenummer2
Anzahl der Seiten18
ISSN1660-3397
DOIs
PublikationsstatusErschienen - 01.02.2022

Bibliographische Notiz

This work was funded by the Latvian ERDF project 1.1.1.1/18/A/022. R.M., M.R.B. and A.P. were supported by University of Latvia under project “Climate change and its impacts on sustainability of natural resources” (Nr. Y5-AZ20-ZF-N-270).

Dokumente

DOI

Zuletzt angesehen

Publikationen

  1. Ulman, Lloyd, Barry Eichengreen and William T. Dickens (eds.)( 1993): Labor and an Integrated Europe, Washington, D.C.: The Brookings Institution, 295 pp. $ 36.95. ISBN 0-8157-8628-4.
  2. Videos oder Texte in der Lehrerbildung? Effekte unterschiedlicher Medien auf die kognitive Belastung und die motivational-emotionalen Prozesse beim Lernen mit Fällen
  3. Implementation of Chemometric Tools to Improve Data Mining and Prioritization in LC-HRMS for Nontarget Screening of Organic Micropollutants in Complex Water Matrixes
  4. Daten des IAB-Betriebspanels und Firmenpaneldaten aus Erhebungen der Amtlichen Statistik - substitutive oder komplementäre Inputs für die Empirische Wirtschaftsforschung?
  5. Acs, Zoltan J. and Audretsch, David B. (eds.): Small Firms and Entrepreneurship: An East-West Perspective, Cambridge/New York: Cambridge University Press, 1993.240 pp. E 30.00. ISBN 0-52143115-8.
  6. Exploring teachers' mental health literacy: An exploratory study on teachers' experiences, cooperation partners, self-efficacy, and knowledge related to students' mental health problems
  7. Polachek, Solomon W. and Siebert, W. Stanley: The Economics of Earnings, Cambridge/New York: Cambridge University Press, 1993.367 pp. E 40.00 (hard). ISBN 0-521-36476-0. E 15.95 (paper). ISBN 0-521-36728-X.
  8. Buechtemann, Christoph F. (ed.): Employment Security and Labor Market Behavior. Interdisciplinary Approaches and International Evidence, Ithaca, N.Y.: ILR Press, 1993.512 pp. $68.00. ISBN 0-87546-188-3.
  9. Rezension: Vico Leuchte (2011). Landkommunen in Ostdeutschland. Lebensgeschichten, Identitätsentfaltung und Sozialwelt.Opladen & Farmington Hills: Verlag Barbara Budrich; 515 Seiten; ISBN 978-3-86649-401-5
  10. REGIONALE VERSORGUNGSSTRUKTUR IM BEREICH DER SUCHTKRANKENHILFE. MODELL EINES GEMEINDENAHEN VERSORGUNGSSYSTEMS UND ERGEBNISSE AUS EINER EMPIRISCHEN UNTERSUCHUNG BEZOGEN AUF STADT UND LANDKREIS LUNEBURG