TY - JOUR

T1 - Kinetic and Stoichiometric Modeling-Based Analysis of Docosahexaenoic Acid (DHA) Production Potential by Crypthecodinium cohnii from Glycerol, Glucose and Ethanol

AU - Berzins, Kristaps

AU - Muiznieks, Reinis

AU - Baumanis, Matiss R.

AU - Strazdina, Inese

AU - Shvirksts, Karlis

AU - Prikule, Santa

AU - Galvanauskas, Vytautas

AU - Pleissner, Daniel

AU - Pentjuss, Agris

AU - Grube, Mara

AU - Kalnenieks, Uldis

AU - Stalidzans, Egils

N1 - This work was funded by the Latvian ERDF project 1.1.1.1/18/A/022. R.M., M.R.B. and A.P. were supported by University of Latvia under project “Climate change and its impacts on sustainability of natural resources” (Nr. Y5-AZ20-ZF-N-270).

PY - 2022/2/1

Y1 - 2022/2/1

N2 - Docosahexaenoic acid (DHA) is one of the most important long-chain polyunsaturated fatty acids (LC-PUFAs), with numerous health benefits. Crypthecodinium cohnii, a marine hetero-trophic dinoflagellate, is successfully used for the industrial production of DHA because it can ac-cumulate DHA at high concentrations within the cells. Glycerol is an interesting renewable substrate for DHA production since it is a by-product of biodiesel production and other industries, and is globally generated in large quantities. The DHA production potential from glycerol, ethanol and glucose is compared by combining fermentation experiments with the pathway-scale kinetic modeling and constraint-based stoichiometric modeling of C. cohnii metabolism. Glycerol has the slow-est biomass growth rate among the tested substrates. This is partially compensated by the highest PUFAs fraction, where DHA is dominant. Mathematical modeling reveals that glycerol has the best experimentally observed carbon transformation rate into biomass, reaching the closest values to the theoretical upper limit. In addition to our observations, the published experimental evidence indi-cates that crude glycerol is readily consumed by C. cohnii, making glycerol an attractive substrate for DHA production.

AB - Docosahexaenoic acid (DHA) is one of the most important long-chain polyunsaturated fatty acids (LC-PUFAs), with numerous health benefits. Crypthecodinium cohnii, a marine hetero-trophic dinoflagellate, is successfully used for the industrial production of DHA because it can ac-cumulate DHA at high concentrations within the cells. Glycerol is an interesting renewable substrate for DHA production since it is a by-product of biodiesel production and other industries, and is globally generated in large quantities. The DHA production potential from glycerol, ethanol and glucose is compared by combining fermentation experiments with the pathway-scale kinetic modeling and constraint-based stoichiometric modeling of C. cohnii metabolism. Glycerol has the slow-est biomass growth rate among the tested substrates. This is partially compensated by the highest PUFAs fraction, where DHA is dominant. Mathematical modeling reveals that glycerol has the best experimentally observed carbon transformation rate into biomass, reaching the closest values to the theoretical upper limit. In addition to our observations, the published experimental evidence indi-cates that crude glycerol is readily consumed by C. cohnii, making glycerol an attractive substrate for DHA production.

KW - Central metabolism

KW - Constraint-based model

KW - FTIR spectroscopy

KW - Kinetic model

KW - Krebs cycle

KW - Biology

UR - http://www.scopus.com/inward/record.url?scp=85123212193&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/486a38c4-538a-3cdb-a672-bcd7b1680e97/

U2 - 10.3390/md20020115

DO - 10.3390/md20020115

M3 - Journal articles

C2 - 35200644

AN - SCOPUS:85123212193

VL - 20

JO - Marine Drugs

JF - Marine Drugs

SN - 1660-3397

IS - 2

M1 - 115

ER -