Rapid antibiotic drug-monitoring: Meropenem and ceftazidime determination in serum and bronchial secretions by high-performance liquid chromatography–integrated sample preparation
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Rapid antibiotic drug-monitoring : Meropenem and ceftazidime determination in serum and bronchial secretions by high-performance liquid chromatography–integrated sample preparation. / Ehrlich, Markus; Daschner, F. D.; Kümmerer, Klaus.
In: Journal of Chromatography B, Vol. 751, No. 2, 25.02.2001, p. 357-363 .Research output: Journal contributions › Journal articles › Research › peer-review
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TY - JOUR
T1 - Rapid antibiotic drug-monitoring
T2 - Meropenem and ceftazidime determination in serum and bronchial secretions by high-performance liquid chromatography–integrated sample preparation
AU - Ehrlich, Markus
AU - Daschner, F. D.
AU - Kümmerer, Klaus
N1 - Funding Information: This work was supported by Zeneca and Glaxo Wellcome.
PY - 2001/2/25
Y1 - 2001/2/25
N2 - A sensitive and rapid HPLC assay for the determination of the beta-lactam antibiotics ceftazidime and meropenem in serum and bronchial secretions is described. HPLC-integrated sample preparation allows direct injection of serum samples without any pretreatment. Sputum samples need only a simple homogenisation and volume measurement but no liquefying reagents are necessary. The inline extraction technique is realized by automatically switching from the extraction column to the analytical column. After the matrix passed the extraction column, the retained analyte is quantitatively transferred to the analytical column where separation by isocratic HPLC is performed. Ceftazidime and meropenem are detected according to their absorption maxima at 258 and 296 nm, respectively. The detection limit of both antibiotics is estimated to be better than 0.5 μg/ml in serum as well as in sputum samples. The described procedure allows determination of the antibiotics within 30-45 min, thereby facilitating drug monitoring in clinical routine.
AB - A sensitive and rapid HPLC assay for the determination of the beta-lactam antibiotics ceftazidime and meropenem in serum and bronchial secretions is described. HPLC-integrated sample preparation allows direct injection of serum samples without any pretreatment. Sputum samples need only a simple homogenisation and volume measurement but no liquefying reagents are necessary. The inline extraction technique is realized by automatically switching from the extraction column to the analytical column. After the matrix passed the extraction column, the retained analyte is quantitatively transferred to the analytical column where separation by isocratic HPLC is performed. Ceftazidime and meropenem are detected according to their absorption maxima at 258 and 296 nm, respectively. The detection limit of both antibiotics is estimated to be better than 0.5 μg/ml in serum as well as in sputum samples. The described procedure allows determination of the antibiotics within 30-45 min, thereby facilitating drug monitoring in clinical routine.
KW - Chemistry
KW - Sputum
KW - Bronchial secretion
KW - Monitoring
UR - http://www.scopus.com/inward/record.url?scp=0035946006&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(00)00504-1
DO - 10.1016/S0378-4347(00)00504-1
M3 - Journal articles
VL - 751
SP - 357
EP - 363
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 2
ER -