New validated liquid chromatographic and chemometrics-assisted UV spectroscopic methods for the determination of two multicomponent cough mixtures in syrup.

Research output: Journal contributionsJournal articlesResearch

Authors

  • Ghada M. Hadad
  • A. El-Gindy
  • Waleed M. M. Mahmoud

Multivariate spectrophotometric calibration and liquid chromatographic (LC) methods were applied to the determination of 2 multicomponent mixtures containing diprophylline, guaiphenesin, methylparaben, and propylparaben (Mixture 1), or Clobutinol, orciprenaline, saccharin sodium, and sodium benzoate (Mixture 2). For the multivariate spectrophotometric calibration methods, principal component regression (PCR) and partial least-squares regression (PLS-1), a calibration set of the mixtures consisting of the components of each mixture was prepared in 0.1 M HCl. Analytical figures of merit such as sensitivity, selectivity, limit of quantitation, and limit of detection were determined for both PLS-1 and PCR. The LC separation was achieved on a reversed-phase C 18 analytical column by using isocratic elution with 20 mM potassium dihydrogen phosphate, pH 3.3-acetonitrile (55 + 45, v/v) as the mobile phase and UV detection at 260 and 220 nm for Mixture 1 and Mixture 2, respectively. The proposed methods were validated and successfully applied to the analysis of pharmaceutical formulations and laboratory-prepared mixtures containing the 2 multicomponent combinations.

Translated title of the contributionNeue validierte Flüssigchromatographie und Chemometrie-unterstützte UV-spektroskopische Methoden für die Bestimmung von zwei mehrere Komponenten enthaltenen Hustensäften in Sirup.
Original languageEnglish
JournalJournal of AOAC International
Volume91
Issue number1
Pages (from-to)39-51
Number of pages13
ISSN1060-3271
DOIs
Publication statusPublished - 01.2008
Externally publishedYes

DOI

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