New validated liquid chromatographic and chemometrics-assisted UV spectroscopic methods for the determination of two multicomponent cough mixtures in syrup.

Research output: Journal contributionsJournal articlesResearch

Authors

  • Ghada M. Hadad
  • A. El-Gindy
  • Waleed M. M. Mahmoud

Multivariate spectrophotometric calibration and liquid chromatographic (LC) methods were applied to the determination of 2 multicomponent mixtures containing diprophylline, guaiphenesin, methylparaben, and propylparaben (Mixture 1), or Clobutinol, orciprenaline, saccharin sodium, and sodium benzoate (Mixture 2). For the multivariate spectrophotometric calibration methods, principal component regression (PCR) and partial least-squares regression (PLS-1), a calibration set of the mixtures consisting of the components of each mixture was prepared in 0.1 M HCl. Analytical figures of merit such as sensitivity, selectivity, limit of quantitation, and limit of detection were determined for both PLS-1 and PCR. The LC separation was achieved on a reversed-phase C 18 analytical column by using isocratic elution with 20 mM potassium dihydrogen phosphate, pH 3.3-acetonitrile (55 + 45, v/v) as the mobile phase and UV detection at 260 and 220 nm for Mixture 1 and Mixture 2, respectively. The proposed methods were validated and successfully applied to the analysis of pharmaceutical formulations and laboratory-prepared mixtures containing the 2 multicomponent combinations.

Translated title of the contributionNeue validierte Flüssigchromatographie und Chemometrie-unterstützte UV-spektroskopische Methoden für die Bestimmung von zwei mehrere Komponenten enthaltenen Hustensäften in Sirup.
Original languageEnglish
JournalJournal of AOAC International
Volume91
Issue number1
Pages (from-to)39-51
Number of pages13
ISSN1060-3271
DOIs
Publication statusPublished - 01.2008
Externally publishedYes

DOI

Recently viewed

Publications

  1. Eco product lifecycle management
  2. Bolt load retention and creep response of AS41 alloyed with 0.15% Ca
  3. Mapping social values of ecosystem services: What is behind the map?
  4. Sustainability performance measurement – a framework for context-specific applications
  5. Tetrabutylammonium prolinate-based ionic liquids
  6. Performance Saga: Interview 07
  7. Preventing Depression in Adults With Subthreshold Depression
  8. Singularities and Superintelligence
  9. A web- And mobile-based intervention for comorbid, recurrent depression in patients with chronic back pain on sick leave (get.back)
  10. Integrating sense of place into participatory landscape planning: merging mapping surveys and geodesign workshops
  11. How does economic integration influence employment and wages in border regions?
  12. Surveying the FAIRness of Annotation Tools: Difficult to find, difficult to reuse
  13. The effect of complacency potential on human operators’ monitoring behavior in aviation
  14. INFLEXIBLE COMMODITY PRICES AS A RESULT OF PROFIT MAXIMIZATION - REPLY TO A COMMENT BY MAUSSNER,A.
  15. How Participatory Should Environmental Governance Be?
  16. Comment on "Recent origin and cultural reversion of a hunter-gatherer group
  17. Using meaningful places as an indicator for sense of place in the management of social-ecological systems
  18. Group membership does not modulate automatic imitation
  19. Klimapaket
  20. "God Is in the Details", or The Filing Box Answers
  21. Introduction
  22. Germany
  23. Vergesellschaftung durch Konsum
  24. Public understanding of climate change terminology in Germany
  25. More than Yield
  26. Verkleben
  27. Einleitung
  28. Bioconversion of agri-food residues into lactic acid
  29. Influence of three different balance shoes on EMG-activity during one-leg standing
  30. Impact and Importance of Heterocyclics in Remediation
  31. Materialities of the Performative
  32. Towards a decision support system for radiotherapy business continuity in a pandemic crisis