Fast, Fully Automated Analysis of Voriconazole from Serum by LC-LC-ESI-MS-MS with Parallel Column-Switching Technique
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In: Journal of Chromatography B, Vol. 814, No. 2, 25.01.2005, p. 361-367.
Research output: Journal contributions › Journal articles › Research › peer-review
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TY - JOUR
T1 - Fast, Fully Automated Analysis of Voriconazole from Serum by LC-LC-ESI-MS-MS with Parallel Column-Switching Technique
AU - Egle, Hannes
AU - Trittler, Rainer
AU - König, Armin
AU - Kümmerer, Klaus
PY - 2005/1/25
Y1 - 2005/1/25
N2 - Voriconazole is a novel broad-spectrum antifungal agent. We developed an on-line LC-LC-MS-MS method for fully automated and direct analysis of voriconazole in raw human serum. After injection of human serum size-selective sample fractionation and analyte extraction was achieved using an extraction column (25 mm × 4 mm) packed with a restricted access material (RAM, LiChrospher® ADS C 8, 25 μm). On-line transfer of voriconazole from the extraction column was followed by chromatography separation on a C 18 column. Detection was done by ESI-MS-MS. The total analysis time was 13 min, managed by parallel extraction and chromatographic separation. This LC-MS assay was fully validated. The lower limit of quantification was 0.05 μg/ml. The automated inline extraction of voriconazole described here eliminates the need for difficult and time-consuming sample pre-treatment. Other advantages of the new method are that only a small quantity (5 μl) of serum is needed and that the method is very specific. © 2004 Elsevier B.V. All rights reserved.
AB - Voriconazole is a novel broad-spectrum antifungal agent. We developed an on-line LC-LC-MS-MS method for fully automated and direct analysis of voriconazole in raw human serum. After injection of human serum size-selective sample fractionation and analyte extraction was achieved using an extraction column (25 mm × 4 mm) packed with a restricted access material (RAM, LiChrospher® ADS C 8, 25 μm). On-line transfer of voriconazole from the extraction column was followed by chromatography separation on a C 18 column. Detection was done by ESI-MS-MS. The total analysis time was 13 min, managed by parallel extraction and chromatographic separation. This LC-MS assay was fully validated. The lower limit of quantification was 0.05 μg/ml. The automated inline extraction of voriconazole described here eliminates the need for difficult and time-consuming sample pre-treatment. Other advantages of the new method are that only a small quantity (5 μl) of serum is needed and that the method is very specific. © 2004 Elsevier B.V. All rights reserved.
KW - Chemistry
KW - Voriconazole
KW - Column-switching
KW - LC–LC–ESI-MS–MS
KW - Serum
KW - On-line sample preparation
UR - http://www.scopus.com/inward/record.url?scp=11844264843&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/25d59003-dea8-3972-94f5-55478265ca81/
U2 - 10.1016/j.jchromb.2004.10.069
DO - 10.1016/j.jchromb.2004.10.069
M3 - Journal articles
C2 - 15639460
VL - 814
SP - 361
EP - 367
JO - Journal of Chromatography B
JF - Journal of Chromatography B
SN - 1873-376X
IS - 2
ER -