TY - JOUR

T1 - Fast, Fully Automated Analysis of Voriconazole from Serum by LC-LC-ESI-MS-MS with Parallel Column-Switching Technique

AU - Egle, Hannes

AU - Trittler, Rainer

AU - König, Armin

AU - Kümmerer, Klaus

PY - 2005/1/25

Y1 - 2005/1/25

N2 - Voriconazole is a novel broad-spectrum antifungal agent. We developed an on-line LC-LC-MS-MS method for fully automated and direct analysis of voriconazole in raw human serum. After injection of human serum size-selective sample fractionation and analyte extraction was achieved using an extraction column (25 mm × 4 mm) packed with a restricted access material (RAM, LiChrospher® ADS C 8, 25 μm). On-line transfer of voriconazole from the extraction column was followed by chromatography separation on a C 18 column. Detection was done by ESI-MS-MS. The total analysis time was 13 min, managed by parallel extraction and chromatographic separation. This LC-MS assay was fully validated. The lower limit of quantification was 0.05 μg/ml. The automated inline extraction of voriconazole described here eliminates the need for difficult and time-consuming sample pre-treatment. Other advantages of the new method are that only a small quantity (5 μl) of serum is needed and that the method is very specific. © 2004 Elsevier B.V. All rights reserved.

AB - Voriconazole is a novel broad-spectrum antifungal agent. We developed an on-line LC-LC-MS-MS method for fully automated and direct analysis of voriconazole in raw human serum. After injection of human serum size-selective sample fractionation and analyte extraction was achieved using an extraction column (25 mm × 4 mm) packed with a restricted access material (RAM, LiChrospher® ADS C 8, 25 μm). On-line transfer of voriconazole from the extraction column was followed by chromatography separation on a C 18 column. Detection was done by ESI-MS-MS. The total analysis time was 13 min, managed by parallel extraction and chromatographic separation. This LC-MS assay was fully validated. The lower limit of quantification was 0.05 μg/ml. The automated inline extraction of voriconazole described here eliminates the need for difficult and time-consuming sample pre-treatment. Other advantages of the new method are that only a small quantity (5 μl) of serum is needed and that the method is very specific. © 2004 Elsevier B.V. All rights reserved.

KW - Chemistry

KW - Voriconazole

KW - Column-switching

KW - LC–LC–ESI-MS–MS

KW - Serum

KW - On-line sample preparation

UR - http://www.scopus.com/inward/record.url?scp=11844264843&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/25d59003-dea8-3972-94f5-55478265ca81/

U2 - 10.1016/j.jchromb.2004.10.069

DO - 10.1016/j.jchromb.2004.10.069

M3 - Journal articles

C2 - 15639460

VL - 814

SP - 361

EP - 367

JO - Journal of Chromatography B

JF - Journal of Chromatography B

SN - 1873-376X

IS - 2

ER -