Ecosystem accounting is receiving increasing interest as a way to systematically monitor the conditions of ecosystems and the ecosystem services they provide. A critical element of ecosystem accounting is understanding spatially explicit flows of ecosystem services. We developed spatial biophysical models of seven ecosystem services in a cultural landscape (Limburg province, the Netherlands) in a way that is consistent with ecosystem accounting. We included hunting, drinking water extraction, crop production, fodder production, air quality regulation, carbon sequestration and recreational cycling. In addition, we examined how human inputs can be distinguished from ecosystem services, a critical element in ecosystem accounting. Model outcomes were used to develop an ecosystem accounting table in line with the System of Environmental-Economic Accounting - Experimental Ecosystem Accounting (SEEA EEA) guidelines, in which contributions of land cover types to ecosystem service flows were recorded. Furthermore we developed spatial accounts for single statistical units. This study shows that for the case of Limburg spatial modelling for ecosystem accounting in line with SEEA EEA is feasible. The paper also analyses and discusses key challenges that need to be addressed to develop a well-functioning system for ecosystem accounting.