Determination of the antifungal agent posaconazole in human serum by HPLC with parallel column-switching technique
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Determination of the antifungal agent posaconazole in human serum by HPLC with parallel column-switching technique. / Neubauer, Werner Christian; König, Armin; Bolek, Richard et al.
In: Journal of Chromatography B, Vol. 877, No. 24, 15.08.2009, p. 2493-2498.Research output: Journal contributions › Journal articles › Research › peer-review
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TY - JOUR
T1 - Determination of the antifungal agent posaconazole in human serum by HPLC with parallel column-switching technique
AU - Neubauer, Werner Christian
AU - König, Armin
AU - Bolek, Richard
AU - Trittler, Rainer
AU - Engelhardt, Monika
AU - Jung, Manfred
AU - Kümmerer, Klaus
PY - 2009/8/15
Y1 - 2009/8/15
N2 - Posaconazole is a new broad-spectrum antifungal agent that is currently only available as an oral suspension and shows high intra- and inter-individual differences in oral bioavailibility. Pre-existing methods for the determination of the substance involve the use of internal standards or require a quite complicated and time-consuming sample pre-treatment. Our HPLC method is fast and fully-automated and there is no need for any manual sample pre-treatment. On-line transfer of posaconazole from the extraction column was followed by chromatographic separation on a C18 column and fluorescence detection (λex: 261 nm, λem: 357 nm). Retention time of posaconazole was about 11.7 min, the lower limit of quantification was found to be 0.1 mg/l. A linear calibration curve was obtained over the concentration range of 0.1-5 mg/l using a 50 μl sample (r2 = 0.999). The relative standard deviations of intra-day variations ranged from 2.3% to 9.4%, intra-day accuracy from 88.8% to 114.8%. © 2009 Elsevier B.V. All rights reserved.
AB - Posaconazole is a new broad-spectrum antifungal agent that is currently only available as an oral suspension and shows high intra- and inter-individual differences in oral bioavailibility. Pre-existing methods for the determination of the substance involve the use of internal standards or require a quite complicated and time-consuming sample pre-treatment. Our HPLC method is fast and fully-automated and there is no need for any manual sample pre-treatment. On-line transfer of posaconazole from the extraction column was followed by chromatographic separation on a C18 column and fluorescence detection (λex: 261 nm, λem: 357 nm). Retention time of posaconazole was about 11.7 min, the lower limit of quantification was found to be 0.1 mg/l. A linear calibration curve was obtained over the concentration range of 0.1-5 mg/l using a 50 μl sample (r2 = 0.999). The relative standard deviations of intra-day variations ranged from 2.3% to 9.4%, intra-day accuracy from 88.8% to 114.8%. © 2009 Elsevier B.V. All rights reserved.
KW - Chemistry
KW - posaconazole
KW - posaconazole
KW - Antifungal Agents
KW - Chromatography, High Pressure Liquid
KW - Humans
KW - Sensitivity and Specificity
KW - Triazoles
KW - Bioavailability
KW - Column-switching
KW - HPLC
KW - On-line sample preparation
KW - Posaconazole
KW - Serum
KW - Bioavailability
KW - Column-switching
KW - HPLC
KW - On-line sample preparation
KW - Posaconazole
KW - Serum
UR - http://www.scopus.com/inward/record.url?scp=67650726438&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/98d7bc4b-7d88-34a5-b87d-6815c07fb563/
U2 - 10.1016/j.jchromb.2009.06.022
DO - 10.1016/j.jchromb.2009.06.022
M3 - Journal articles
C2 - 19577524
VL - 877
SP - 2493
EP - 2498
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 24
ER -