TY - JOUR

T1 - Determination of the antifungal agent posaconazole in human serum by HPLC with parallel column-switching technique

AU - Neubauer, Werner Christian

AU - König, Armin

AU - Bolek, Richard

AU - Trittler, Rainer

AU - Engelhardt, Monika

AU - Jung, Manfred

AU - Kümmerer, Klaus

PY - 2009/8/15

Y1 - 2009/8/15

N2 - Posaconazole is a new broad-spectrum antifungal agent that is currently only available as an oral suspension and shows high intra- and inter-individual differences in oral bioavailibility. Pre-existing methods for the determination of the substance involve the use of internal standards or require a quite complicated and time-consuming sample pre-treatment. Our HPLC method is fast and fully-automated and there is no need for any manual sample pre-treatment. On-line transfer of posaconazole from the extraction column was followed by chromatographic separation on a C18 column and fluorescence detection (λex: 261 nm, λem: 357 nm). Retention time of posaconazole was about 11.7 min, the lower limit of quantification was found to be 0.1 mg/l. A linear calibration curve was obtained over the concentration range of 0.1-5 mg/l using a 50 μl sample (r2 = 0.999). The relative standard deviations of intra-day variations ranged from 2.3% to 9.4%, intra-day accuracy from 88.8% to 114.8%. © 2009 Elsevier B.V. All rights reserved.

AB - Posaconazole is a new broad-spectrum antifungal agent that is currently only available as an oral suspension and shows high intra- and inter-individual differences in oral bioavailibility. Pre-existing methods for the determination of the substance involve the use of internal standards or require a quite complicated and time-consuming sample pre-treatment. Our HPLC method is fast and fully-automated and there is no need for any manual sample pre-treatment. On-line transfer of posaconazole from the extraction column was followed by chromatographic separation on a C18 column and fluorescence detection (λex: 261 nm, λem: 357 nm). Retention time of posaconazole was about 11.7 min, the lower limit of quantification was found to be 0.1 mg/l. A linear calibration curve was obtained over the concentration range of 0.1-5 mg/l using a 50 μl sample (r2 = 0.999). The relative standard deviations of intra-day variations ranged from 2.3% to 9.4%, intra-day accuracy from 88.8% to 114.8%. © 2009 Elsevier B.V. All rights reserved.

KW - Chemistry

KW - posaconazole

KW - posaconazole

KW - Antifungal Agents

KW - Chromatography, High Pressure Liquid

KW - Humans

KW - Sensitivity and Specificity

KW - Triazoles

KW - Bioavailability

KW - Column-switching

KW - HPLC

KW - On-line sample preparation

KW - Posaconazole

KW - Serum

KW - Bioavailability

KW - Column-switching

KW - HPLC

KW - On-line sample preparation

KW - Posaconazole

KW - Serum

UR - http://www.scopus.com/inward/record.url?scp=67650726438&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/98d7bc4b-7d88-34a5-b87d-6815c07fb563/

U2 - 10.1016/j.jchromb.2009.06.022

DO - 10.1016/j.jchromb.2009.06.022

M3 - Journal articles

C2 - 19577524

VL - 877

SP - 2493

EP - 2498

JO - Journal of Chromatography B

JF - Journal of Chromatography B

SN - 1873-376X

IS - 24

ER -