Combined MRI-PET dissects dynamic changes in plant structures and functions
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In: Plant Journal, Vol. 59, No. 4, 01.08.2009, p. 634-644.
Research output: Journal contributions › Journal articles › Research › peer-review
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TY - JOUR
T1 - Combined MRI-PET dissects dynamic changes in plant structures and functions
AU - Jahnke, Siegfried
AU - Menzel, Marion I.
AU - Van Dusschoten, Dagmar
AU - Roeb, Gerhard W.
AU - Bühler, Jonas
AU - Minwuyelet, Senay
AU - Blümler, Peter
AU - Temperton, Vicky M.
AU - Hombach, Thomas
AU - Streun, Matthias
AU - Beer, Simone
AU - Khodaverdi, Maryam
AU - Ziemons, Karl
AU - Coenen, Heinz H.
AU - Schurr, Ulrich
PY - 2009/8/1
Y1 - 2009/8/1
N2 - Unravelling the factors determining the allocation of carbon to various plant organs is one of the great challenges of modern plant biology. Studying allocation under close to natural conditions requires non-invasive methods, which are now becoming available for measuring plants on a par with those developed for humans. By combining magnetic resonance imaging (MRI) and positron emission tomography (PET), we investigated three contrasting root/shoot systems growing in sand or soil, with respect to their structures, transport routes and the translocation dynamics of recently fixed photoassimilates labelled with the short-lived radioactive carbon isotope 11C. Storage organs of sugar beet (Beta vulgaris) and radish plants (Raphanus sativus) were assessed using MRI, providing images of the internal structures of the organs with high spatial resolution, and while species-specific transport sectoralities, properties of assimilate allocation and unloading characteristics were measured using PET. Growth and carbon allocation within complex root systems were monitored in maize plants (Zea mays), and the results may be used to identify factors affecting root growth in natural substrates or in competition with roots of other plants. MRI-PET co-registration opens the door for non-invasive analysis of plant structures and transport processes that may change in response to genomic, developmental or environmental challenges. It is our aim to make the methods applicable for quantitative analyses of plant traits in phenotyping as well as in understanding the dynamics of key processes that are essential to plant performance.
AB - Unravelling the factors determining the allocation of carbon to various plant organs is one of the great challenges of modern plant biology. Studying allocation under close to natural conditions requires non-invasive methods, which are now becoming available for measuring plants on a par with those developed for humans. By combining magnetic resonance imaging (MRI) and positron emission tomography (PET), we investigated three contrasting root/shoot systems growing in sand or soil, with respect to their structures, transport routes and the translocation dynamics of recently fixed photoassimilates labelled with the short-lived radioactive carbon isotope 11C. Storage organs of sugar beet (Beta vulgaris) and radish plants (Raphanus sativus) were assessed using MRI, providing images of the internal structures of the organs with high spatial resolution, and while species-specific transport sectoralities, properties of assimilate allocation and unloading characteristics were measured using PET. Growth and carbon allocation within complex root systems were monitored in maize plants (Zea mays), and the results may be used to identify factors affecting root growth in natural substrates or in competition with roots of other plants. MRI-PET co-registration opens the door for non-invasive analysis of plant structures and transport processes that may change in response to genomic, developmental or environmental challenges. It is our aim to make the methods applicable for quantitative analyses of plant traits in phenotyping as well as in understanding the dynamics of key processes that are essential to plant performance.
KW - Carbon-11 (C)
KW - Co-registration
KW - Functional imaging (3D)
KW - Magnetic resonance imaging (MRI)
KW - Non-invasive method
KW - Positron emission tomography (PET)
KW - Biology
KW - Ecosystems Research
UR - http://www.scopus.com/inward/record.url?scp=68849114351&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/0fba4b7f-5783-37a4-b44f-b64346ac993f/
U2 - 10.1111/j.1365-313X.2009.03888.x
DO - 10.1111/j.1365-313X.2009.03888.x
M3 - Journal articles
C2 - 19392708
AN - SCOPUS:68849114351
VL - 59
SP - 634
EP - 644
JO - Plant Journal
JF - Plant Journal
SN - 0960-7412
IS - 4
ER -