Quantification of amino acids in fermentation media by isocratic HPLC analysis of their

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschungbegutachtet

Standard

Quantification of amino acids in fermentation media by isocratic HPLC analysis of their. / Pleissner, Daniel; Wimmer, Reinhard; Eriksen, Niels T.
in: Analytical Chemistry, Jahrgang 83, Nr. 1, 01.01.2011, S. 175-181.

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschungbegutachtet

Harvard

APA

Vancouver

Bibtex

@article{60d15eb2dbdb4f77a6ad91186929a639,
title = "Quantification of amino acids in fermentation media by isocratic HPLC analysis of their",
abstract = "In this paper we describe a novel method for quantification of amino acids. First, α-hydroxy acid derivatives of amino acids were formed after reaction with dinitrogen trioxide by the van Slyke reaction. Second, the α-hydroxy acid derivatives were separated on an Aminex HPX-87H column (Bio-Rad) eluted isocratically with 5 mM H2SO4 and quantified by refractive index detection. We were able to measure the reaction products of 13 of the 20 classical amino acids: glycine, l-alanine, l-valine, l-leucine, l-isoleucine, l-methionine, l-serine, l-threonine, l-asparagine, l-glutamine, l-aspartic acid, l-glutamic acid, and l-proline. We obtained linear relationships between the product peak areas and initial amino acid concentration, whereby the concentrations of these amino acids could be quantified on the basis of the quantification of their products. The method can be used to analyze amino acids in parallel with other small molecules, such as sugars or short chain fatty acids, and was used for parallel quantification of glycine, l-alanine, or l-glutamic acid, and glucose uptake in cultures of the heterotrophic dinoflagellate Crypthecodinium cohnii. The method can also be used to quantify other amines, as demonstrated by detection of Tris (2-amino-2-(hydroxymethyl)propane-1,3-diol).",
keywords = "Chemistry",
author = "Daniel Pleissner and Reinhard Wimmer and Eriksen, {Niels T.}",
year = "2011",
month = jan,
day = "1",
doi = "10.1021/ac1021908",
language = "English",
volume = "83",
pages = "175--181",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "1",

}

RIS

TY - JOUR

T1 - Quantification of amino acids in fermentation media by isocratic HPLC analysis of their

AU - Pleissner, Daniel

AU - Wimmer, Reinhard

AU - Eriksen, Niels T.

PY - 2011/1/1

Y1 - 2011/1/1

N2 - In this paper we describe a novel method for quantification of amino acids. First, α-hydroxy acid derivatives of amino acids were formed after reaction with dinitrogen trioxide by the van Slyke reaction. Second, the α-hydroxy acid derivatives were separated on an Aminex HPX-87H column (Bio-Rad) eluted isocratically with 5 mM H2SO4 and quantified by refractive index detection. We were able to measure the reaction products of 13 of the 20 classical amino acids: glycine, l-alanine, l-valine, l-leucine, l-isoleucine, l-methionine, l-serine, l-threonine, l-asparagine, l-glutamine, l-aspartic acid, l-glutamic acid, and l-proline. We obtained linear relationships between the product peak areas and initial amino acid concentration, whereby the concentrations of these amino acids could be quantified on the basis of the quantification of their products. The method can be used to analyze amino acids in parallel with other small molecules, such as sugars or short chain fatty acids, and was used for parallel quantification of glycine, l-alanine, or l-glutamic acid, and glucose uptake in cultures of the heterotrophic dinoflagellate Crypthecodinium cohnii. The method can also be used to quantify other amines, as demonstrated by detection of Tris (2-amino-2-(hydroxymethyl)propane-1,3-diol).

AB - In this paper we describe a novel method for quantification of amino acids. First, α-hydroxy acid derivatives of amino acids were formed after reaction with dinitrogen trioxide by the van Slyke reaction. Second, the α-hydroxy acid derivatives were separated on an Aminex HPX-87H column (Bio-Rad) eluted isocratically with 5 mM H2SO4 and quantified by refractive index detection. We were able to measure the reaction products of 13 of the 20 classical amino acids: glycine, l-alanine, l-valine, l-leucine, l-isoleucine, l-methionine, l-serine, l-threonine, l-asparagine, l-glutamine, l-aspartic acid, l-glutamic acid, and l-proline. We obtained linear relationships between the product peak areas and initial amino acid concentration, whereby the concentrations of these amino acids could be quantified on the basis of the quantification of their products. The method can be used to analyze amino acids in parallel with other small molecules, such as sugars or short chain fatty acids, and was used for parallel quantification of glycine, l-alanine, or l-glutamic acid, and glucose uptake in cultures of the heterotrophic dinoflagellate Crypthecodinium cohnii. The method can also be used to quantify other amines, as demonstrated by detection of Tris (2-amino-2-(hydroxymethyl)propane-1,3-diol).

KW - Chemistry

UR - http://www.scopus.com/inward/record.url?scp=78650783616&partnerID=8YFLogxK

U2 - 10.1021/ac1021908

DO - 10.1021/ac1021908

M3 - Journal articles

C2 - 21121687

VL - 83

SP - 175

EP - 181

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 1

ER -

DOI

Zuletzt angesehen

Forschende

  1. Joyce Müller

Aktivitäten

  1. Von Foerster‘s chap does brainy job. Die Prototypen des Biological Computer Laboratory (Hyperkult 18)
  2. Correlation Patterns of PAHs and Heterocyclic PAHs in Sediment Samples from Influence of Two Tar-Contaminated Sites on Adjacent Rivers: Quantitation of PAHs, Heterocyclic PAHs and Phenols in the Aqueous Phase
  3. Efficiency through Democracy? Concepts and Evidence from Environmental Governance (with O. Fritsch)
  4. Co-Supervisor for the dissertation "Biodiversity and functional genetic connectivity of ground-dwelling insects"
  5. 12. Tag der Hydrologie - 2010 (Veranstaltung)
  6. Intraorganizational tensions around being good: Structures and practices of organizing purchasing and CSR in garment retailers and brands
  7. Aarhus Universität
  8. Guest lecture Carbon performance and disclosure: Governance-related determinants and their firms’ financial consequences
  9. Journal of Applied Ecology (Fachzeitschrift)
  10. EURISY: Models of Governance of National Space Activities in the Evolving European Framework 2009
  11. New Space Services on Earth
  12. Methoden transformativer Forschung
  13. Fakultät Management und Technologie (Organisation)
  14. Association for Information Systems (AIS) (Externe Organisation)
  15. DFG-Gutachtertätigkeit
  16. Network Governance and Collective Learning in Environmental Management (with D. Günther and C. Pahl-Wostl)
  17. International Relations (Fachzeitschrift)
  18. Network Governance, Social Learning and Sustainability Transitions (with D. Günther)
  19. Academy of Management (Externe Organisation)
  20. Life cycle engineering (LCE) of pharmaceuticals: systematic optimization for use as well as the environment.
  21. Fostering Creative Processes through „Challenge“ and „Collaboration

Publikationen

  1. Same but different? Measurement invariance of the PIAAC motivation-to-learn scale across key socio-demographic groups
  2. Adapting videogame interfaces for the visually impaired
  3. Design of finger joint implants based on triply periodic minimal surfaces
  4. Introduction - Teaching Artistic Strategies. Playing with Materiality, Aesthetics and Ambiguity
  5. Article 11 Formal Validity
  6. When Do Pictures Help Learning from Expository Text? Multimedia and Modality Effects in Primary Schools
  7. Ringen um Sinn
  8. Information rigidities, inflation perceptions, and the media
  9. The u-shape on aging revisited
  10. Ecosystem services as a boundary object for sustainability
  11. Exceeding Work
  12. Reconstructing Diversity Management and Communication from a Constitutive-Polyphonic Perspective
  13. Where pragmatics and dialectology meet
  14. Learning to rank user intent
  15. Christian Steinbacher
  16. A high power dye laser pumped by a crowbar mode flashlamp
  17. Comparing Instrument-induced effects in EFL requests
  18. CAN BUSINESS MODEL COMPONENTS EXPLAIN DIGITAL START-UP SUCCESS?
  19. Citizen Action in the Time of the Network
  20. The New Media
  21. Two high-mountain burnet moth species (Lepidoptera, Zygaenidae) react differently to the global change drivers climate and land-use
  22. Variation in short-term and long-term responses of photosynthesis and isoprenoid-mediated photoprotection to soil water availability in four Douglas-fir provenances
  23. Harmonization in the World Values Survey
  24. Microstructure and creep properties of MEZ magnesium alloy processed by thixocasting
  25. Future Challenges for Global Tourism