To achieve successful autonomous navigation, a vision system that provides continuous and precise three-dimensional data of the system’s surroundings is always needed. An indispensable step in the acquisition of reliable data is the calibration of the system, preferably with a time-efficient and low-complexity approach. In this paper, a robust and efficient calibration method is proposed for the information fusion of a stereo vision system and a Technical Vision System. The proposed methodology achieves an error of xe = 6.9401 mm, ye = 8.0997 mm and ze = 15.1822 mm in 3.0202 seconds of processing time, as proven through experimental results.