TY - JOUR

T1 - Enhancement of cellular immune response to a prostate cancer DNA vaccine by intradermal electroporation.

AU - Roos, Anna-Karin

AU - Moreno, Sonia

AU - Leder, Christoph

AU - Pavlenko, Maxim

AU - King, Alan

AU - Pisa, Pavel

N1 - Funding Information: This work was supported in part by the Cancer Society in Stockholm, the Karolinska Institutes Fund, the Swedish Cancer Society, the EU 6-FP ALLOSTEM (LSHB-CT-2004-502219), and the U.S. Department of Defense Prostate Cancer Research Program (PC030958).

PY - 2006/2/1

Y1 - 2006/2/1

N2 - Recently it has become clear that more potent methods for DNA vaccine delivery need to be developed to enhance the efficacy of DNA vaccines. In vivo electroporation has emerged as a potent method for DNA vaccine delivery. In a mouse model, we evaluated the CD8(+) T lymphocyte response to a prostate cancer DNA vaccine encoding prostate-specific antigen (PSA) after intradermal electroporation. A significantly increased gene expression (100- to 1000-fold) and higher levels of PSA-specific T cells, compared to DNA delivery without electroporation, was demonstrated. Interestingly, investigation of a panel of different electroporation conditions showed that only some conditions that induce high levels of gene expression additionally induced cellular immunity. This suggests that electroporation parameters should be carefully optimized, not only to enhance transfection efficiency, but also to enhance the immune response to the vaccine. This study demonstrates the applicability of intradermal electroporation as a delivery method for genetic cancer vaccines and other DNA vaccines relying on antigen-specific T cell induction.

AB - Recently it has become clear that more potent methods for DNA vaccine delivery need to be developed to enhance the efficacy of DNA vaccines. In vivo electroporation has emerged as a potent method for DNA vaccine delivery. In a mouse model, we evaluated the CD8(+) T lymphocyte response to a prostate cancer DNA vaccine encoding prostate-specific antigen (PSA) after intradermal electroporation. A significantly increased gene expression (100- to 1000-fold) and higher levels of PSA-specific T cells, compared to DNA delivery without electroporation, was demonstrated. Interestingly, investigation of a panel of different electroporation conditions showed that only some conditions that induce high levels of gene expression additionally induced cellular immunity. This suggests that electroporation parameters should be carefully optimized, not only to enhance transfection efficiency, but also to enhance the immune response to the vaccine. This study demonstrates the applicability of intradermal electroporation as a delivery method for genetic cancer vaccines and other DNA vaccines relying on antigen-specific T cell induction.

KW - Biology

KW - electroporation

KW - Prostate-specific antigen

KW - DNA vaccine

KW - CTL

KW - immunotherapy

UR - http://www.scopus.com/inward/record.url?scp=30344433748&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/52c38cd3-dedb-3239-8483-bfe43f590098/

U2 - 10.1016/j.ymthe.2005.08.005

DO - 10.1016/j.ymthe.2005.08.005

M3 - Journal articles

VL - 13

SP - 320

EP - 327

JO - Molecular Therapy

JF - Molecular Therapy

SN - 1525-0024

IS - 2

ER -