Determination of sulfur and selected trace elements in metallothionein-like proteins using capillary electrophoresis hyphenated to inductively coupled plasma mass spectrometry with an octopole reaction cell

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschung

Authors

The determination of sulfur in biologically relevant samples such as metalloproteins is described. The analytical methodology used is based on robust on-line coupling between capillary electrophoresis (CE) and octopole reaction cell inductively-coupled plasma mass spectrometry (ORC-ICP-MS). Polyatomic ions that form in the plasma and interfere with the determination of S at mass 32 are minimised by addition of xenon to the collision cell. The method has been applied to the separation and simultaneous element-specific detection of sulfur, cadmium, copper, and zinc in commercially available metallothionein preparations (MT) and metallothionein-like proteins (MLP) extracted from liver samples of bream (Abramis brama L.) caught in the river Elbe, Germany. Instrumental detection limits have been calculated according to the German standard procedure DIN 32645 for the determination of sulfur and some simultaneously measured trace elements in aqueous solution. For sulfur detection limits down to 1.3μg L -1 ( 34S) and 3.2μg L -1 ( 32S) were derived. For the other trace elements determined simultaneously detection limits ranging from 300ng L -1 ( 58Ni) to 500ng L -1 ( 66Zn, 55Mn) were achieved. For quantification of sulfur and cadmium in a commercially available MT preparation under hyphenated conditions the use of external calibration is suggested. Finally, the need for proper sample-preparation technique will be discussed.

OriginalspracheEnglisch
ZeitschriftAnalytical & Bioanalytical Chemistry
Jahrgang377
Ausgabenummer1
Seiten (von - bis)132-139
Anzahl der Seiten8
ISSN1618-2642
DOIs
PublikationsstatusErschienen - 01.09.2003
Extern publiziertJa

Links

DOI

Zuletzt angesehen

Publikationen

  1. Between morality and the law
  2. Früherkennung als Problem der Unternehmensführung in virtuellen Organisationen
  3. Editorial
  4. Deliberative Bürgerbeteiligung in der Priorisierungsdebatte
  5. Biodiversity–stability relationships strengthen over time in a long-term grassland experiment
  6. Eating stuff found on the floor is good for you: academia takes media non grata under her wings.
  7. How to Integrate Refugees Into the Workforce - Different Opportunities for (Social) Entrepreneurship
  8. Tsunami
  9. Ein neuer Klassenkampf?
  10. Practice What You Preach?
  11. Austria
  12. Institutional challenges for space activities in Europe
  13. Too Scared to Fight Back? Affective Job Insecurity as a Boundary Condition Between Workplace Incivility and Negative Mood States in Temporary Agency Workers
  14. Farewell to the party model?
  15. Beyond the Looking Glass, into the Web
  16. Kontextualisierungshinweise
  17. Nachhaltige Entwicklung
  18. Re-investigating the insurance-growth nexus using common factors
  19. Liability rule failures? Evidence from german court decisions.
  20. The Concept of Personal Initiative
  21. Transformation of Seafood Side-Streams and Residuals into Valuable Products
  22. Wozu KMU-Management
  23. The influence of travel company reputation on online travel decisions
  24. Integration by case, place and process: transdisciplinary research for sustainable grazing in the Lachlan River catchment, Australia
  25. Investigating the Dual Role of Price on Consumers’ Purchase Intentions of Hedonic versus Utilitarian Products:
  26. Making Sociological Theories Come Alive
  27. Ausgewählte Kapitel der Theoretischen Informatik
  28. Taking Care of History
  29. Didaktik privater Rechtsgestaltung
  30. Time Series Explorer
  31. Training Moral Sensitivity Through Video Games
  32. Empirie als Problem?
  33. How to plan as a small scale business owner
  34. Internationaler Masterstudiengang 'Sustainable Development and Management'