A Sensitive Microsystem as Biosensor for Cell Growth Monitoring and Antibiotic Testing

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschungbegutachtet

Standard

A Sensitive Microsystem as Biosensor for Cell Growth Monitoring and Antibiotic Testing. / Spiller, E.; Schöll, A.; Alexy, Radka et al.
in: Sensors and Actuators A: Physical, Jahrgang 130-131, Nr. SPEC. ISS., 14.08.2006, S. 312-321.

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschungbegutachtet

Harvard

APA

Vancouver

Spiller E, Schöll A, Alexy R, Kümmerer K, Urban GA. A Sensitive Microsystem as Biosensor for Cell Growth Monitoring and Antibiotic Testing. Sensors and Actuators A: Physical. 2006 Aug 14;130-131(SPEC. ISS.):312-321. doi: 10.1016/j.sna.2006.02.051

Bibtex

@article{dd4bce8d26bc4aa5bf69697444b75c61,
title = "A Sensitive Microsystem as Biosensor for Cell Growth Monitoring and Antibiotic Testing",
abstract = "Standard bacterial tests such as growth inhibition tests are the only convenient investigation method to detect the impacts of water soluble drugs, e.g. antibiotics, on bacteria. State of the art methods for determining the inhibiting effect of toxic components are cumbersome, because they are for parallelization not feasible. The principle of these inhibition tests is the detection of cell numbers by measuring the optical density. With this time-consuming and complicate method only endpoint detection and no monitoring of cell growth is possible, which is obviously a drawback. Furthermore, colored and turbid components cannot be tested. A new microsystem is introduced in this paper overcoming the mention problems. A continuously working conductivity sensor system for high throughput application is developed. With this miniaturized system a reduction of sample volume from 50 ml down to 12 μl becomes possible. An additional advantage is the possibility of online monitoring during biological tests and cell growth detection in high turbid or colored samples, which could not be investigated before. The sensor system is calibrated for growth inhibition of Pseudomonas putida, tested with antibiotics. {\textcopyright} 2006 Elsevier B.V. All rights reserved.",
keywords = "Antibiotic testing, Cell growth monitoring, High throughput screening, Pseudomonas putida",
author = "E. Spiller and A. Sch{\"o}ll and Radka Alexy and Klaus K{\"u}mmerer and Urban, {G. A.}",
note = "Themenheft: Selected Papers from TRANSDUCERS '05 - The 13th International Conference on Solid-State Sensors, Actuators and Microsystems - Seoul, Korea, 5-9 June 2005 / ed. by P. French",
year = "2006",
month = aug,
day = "14",
doi = "10.1016/j.sna.2006.02.051",
language = "English",
volume = "130-131",
pages = "312--321",
journal = "Sensors and Actuators A: Physical",
issn = "0924-4247",
publisher = "Elsevier B.V.",
number = "SPEC. ISS.",

}

RIS

TY - JOUR

T1 - A Sensitive Microsystem as Biosensor for Cell Growth Monitoring and Antibiotic Testing

AU - Spiller, E.

AU - Schöll, A.

AU - Alexy, Radka

AU - Kümmerer, Klaus

AU - Urban, G. A.

N1 - Themenheft: Selected Papers from TRANSDUCERS '05 - The 13th International Conference on Solid-State Sensors, Actuators and Microsystems - Seoul, Korea, 5-9 June 2005 / ed. by P. French

PY - 2006/8/14

Y1 - 2006/8/14

N2 - Standard bacterial tests such as growth inhibition tests are the only convenient investigation method to detect the impacts of water soluble drugs, e.g. antibiotics, on bacteria. State of the art methods for determining the inhibiting effect of toxic components are cumbersome, because they are for parallelization not feasible. The principle of these inhibition tests is the detection of cell numbers by measuring the optical density. With this time-consuming and complicate method only endpoint detection and no monitoring of cell growth is possible, which is obviously a drawback. Furthermore, colored and turbid components cannot be tested. A new microsystem is introduced in this paper overcoming the mention problems. A continuously working conductivity sensor system for high throughput application is developed. With this miniaturized system a reduction of sample volume from 50 ml down to 12 μl becomes possible. An additional advantage is the possibility of online monitoring during biological tests and cell growth detection in high turbid or colored samples, which could not be investigated before. The sensor system is calibrated for growth inhibition of Pseudomonas putida, tested with antibiotics. © 2006 Elsevier B.V. All rights reserved.

AB - Standard bacterial tests such as growth inhibition tests are the only convenient investigation method to detect the impacts of water soluble drugs, e.g. antibiotics, on bacteria. State of the art methods for determining the inhibiting effect of toxic components are cumbersome, because they are for parallelization not feasible. The principle of these inhibition tests is the detection of cell numbers by measuring the optical density. With this time-consuming and complicate method only endpoint detection and no monitoring of cell growth is possible, which is obviously a drawback. Furthermore, colored and turbid components cannot be tested. A new microsystem is introduced in this paper overcoming the mention problems. A continuously working conductivity sensor system for high throughput application is developed. With this miniaturized system a reduction of sample volume from 50 ml down to 12 μl becomes possible. An additional advantage is the possibility of online monitoring during biological tests and cell growth detection in high turbid or colored samples, which could not be investigated before. The sensor system is calibrated for growth inhibition of Pseudomonas putida, tested with antibiotics. © 2006 Elsevier B.V. All rights reserved.

KW - Antibiotic testing

KW - Cell growth monitoring

KW - High throughput screening

KW - Pseudomonas putida

UR - http://www.scopus.com/inward/record.url?scp=33745858301&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/2fa4e75b-25bf-3e62-afa9-6cc2e4aa3785/

U2 - 10.1016/j.sna.2006.02.051

DO - 10.1016/j.sna.2006.02.051

M3 - Journal articles

VL - 130-131

SP - 312

EP - 321

JO - Sensors and Actuators A: Physical

JF - Sensors and Actuators A: Physical

SN - 0924-4247

IS - SPEC. ISS.

ER -

DOI

Zuletzt angesehen

Publikationen

  1. Working memory capacity and narrative task performance
  2. “Smart is not smart enough!” Anticipating critical raw material use in smart city concepts
  3. From teacher-centered instruction to peer tutoring in the heterogeneous international classroom
  4. Predator diversity and abundance provide little support for the enemies hypothesis in forests of high tree diversity
  5. Tracing Concepts
  6. Introduction
  7. Maschinenbelegungsplanung mit evolutionären Algorithmen
  8. Cross-hedging minimum return guarantees
  9. Atmospheric gas-particle partitioning versus gaseous/particle-bound deposition of SVOCs
  10. Importance of timing
  11. Article 21 Formal Validity
  12. Facing complex crime
  13. Mouseology – Ludic Interfaces – Zero Interfaces
  14. A(l)gora: the Mindscape
  15. Local Responses to Global Integration in a Transnational Professional Service Firm
  16. A qualitative approach to evidence-based entrepreneurship: Theoretical considerations and an example involving business clusters
  17. Fluorometer controlled apparatus designed for long-duration algal-feeding experiments and environmental effect studies with mussels
  18. The mediating role of entrepreneurial orientation in the task-environment-performance relationship
  19. The Plane of Obscurity — Simulation and Philosophy
  20. Towards a Multi-Level Approach to Studying Entrepreneurship in Professional Services
  21. Relationalität I+II
  22. A panel cointegration rank test with structural breaks and cross-sectional dependence
  23. Between the Front Lines
  24. Article 1 Scope
  25. The pace of range expansion
  26. Alcohol Breeds Empty Goal Commitments