Quantitative gas chromatography - mass spectrometry profiling of volatile organic compounds produced by barley (Hordeum distichon L.) roots according to plant age

Research output: Contributions to collected editions/worksPublished abstract in conference proceedingsResearch

Standard

Quantitative gas chromatography - mass spectrometry profiling of volatile organic compounds produced by barley (Hordeum distichon L.) roots according to plant age. / Delory, Benjamin; Delaplace, Pierre; du Jardin, Patrick et al.
Book of short abstracts, Poster presentations: 19th National Symposium on Applied Biological Sciences. Gembloux Agro-Bio Tech, 2014. p. 82.

Research output: Contributions to collected editions/worksPublished abstract in conference proceedingsResearch

Harvard

Delory, B, Delaplace, P, du Jardin, P & Fauconnier, M-L 2014, Quantitative gas chromatography - mass spectrometry profiling of volatile organic compounds produced by barley (Hordeum distichon L.) roots according to plant age. in Book of short abstracts, Poster presentations: 19th National Symposium on Applied Biological Sciences. Gembloux Agro-Bio Tech, pp. 82, 19th National Symposium on Applied Biological Sciences - NSABS 2014, Gembloux, Belgium, 07.02.14.

APA

Delory, B., Delaplace, P., du Jardin, P., & Fauconnier, M.-L. (2014). Quantitative gas chromatography - mass spectrometry profiling of volatile organic compounds produced by barley (Hordeum distichon L.) roots according to plant age. In Book of short abstracts, Poster presentations: 19th National Symposium on Applied Biological Sciences (pp. 82). Gembloux Agro-Bio Tech.

Vancouver

Delory B, Delaplace P, du Jardin P, Fauconnier ML. Quantitative gas chromatography - mass spectrometry profiling of volatile organic compounds produced by barley (Hordeum distichon L.) roots according to plant age. In Book of short abstracts, Poster presentations: 19th National Symposium on Applied Biological Sciences. Gembloux Agro-Bio Tech. 2014. p. 82

Bibtex

@inbook{d6a8489672b54465a029c54e3242af4f,
title = "Quantitative gas chromatography - mass spectrometry profiling of volatile organic compounds produced by barley (Hordeum distichon L.) roots according to plant age",
abstract = "In chemical ecology, the roles played by root-emitted volatile organic compounds (VOCs) in biotic interactions and the quantitative analysis of such chemicals in root tissues remain poorly documented. In this context, this study aims at developing a fully automated analytical methodology allowing both identification and accurate quantification of VOCs produced by roots of a monocotyledon plant species. Briefly, VOC emitted by crushed barley roots are successively trapped by dynamic headspace sampling on Tenax TA adsorbents, thermally desorbed and cryofocused, separated by gas chromatography (GC) and finally analysed by mass spectrometry (MS) in both SCAN and selected ion monitoring modes. Results show that barley roots mainly produce four volatile aldehydes, namely hexanal, (E)-hex-2-enal, (E)-non-2-enal and (E,Z)-nona-2,6-dienal. These molecules are well-known linoleic (C18:2) and linolenic (C18:3) acid derivatives produced via the lipoxygenase and the hydroperoxide lyase pathways of higher plants. Our findings contrast with analyses documented on aboveground barley tissues that mainly emit C6 aldehydes, alcohols and their derivative esters. Moreover, preliminary results indicate quantitative changes in the volatile profile contained in barley roots according to plant age. Multivariate statistical analyses are currently underway to quantitatively assess these changes using plants at five selected developmental stages ranging from germination to the end of tillering.",
keywords = "Biology, Chemistry",
author = "Benjamin Delory and Pierre Delaplace and {du Jardin}, Patrick and Marie-Laure Fauconnier",
year = "2014",
month = feb,
day = "7",
language = "English",
pages = "82",
booktitle = "Book of short abstracts, Poster presentations",
publisher = "Gembloux Agro-Bio Tech",
address = "Belgium",
note = "19th National Symposium on Applied Biological Sciences - NSABS 2014, NSABS 2014 ; Conference date: 07-02-2014 Through 07-02-2014",
url = "http://www.events.gembloux.ulg.ac.be/nsabs2014/nsabs-2014/",

}

RIS

TY - CHAP

T1 - Quantitative gas chromatography - mass spectrometry profiling of volatile organic compounds produced by barley (Hordeum distichon L.) roots according to plant age

AU - Delory, Benjamin

AU - Delaplace, Pierre

AU - du Jardin, Patrick

AU - Fauconnier, Marie-Laure

N1 - Conference code: 19

PY - 2014/2/7

Y1 - 2014/2/7

N2 - In chemical ecology, the roles played by root-emitted volatile organic compounds (VOCs) in biotic interactions and the quantitative analysis of such chemicals in root tissues remain poorly documented. In this context, this study aims at developing a fully automated analytical methodology allowing both identification and accurate quantification of VOCs produced by roots of a monocotyledon plant species. Briefly, VOC emitted by crushed barley roots are successively trapped by dynamic headspace sampling on Tenax TA adsorbents, thermally desorbed and cryofocused, separated by gas chromatography (GC) and finally analysed by mass spectrometry (MS) in both SCAN and selected ion monitoring modes. Results show that barley roots mainly produce four volatile aldehydes, namely hexanal, (E)-hex-2-enal, (E)-non-2-enal and (E,Z)-nona-2,6-dienal. These molecules are well-known linoleic (C18:2) and linolenic (C18:3) acid derivatives produced via the lipoxygenase and the hydroperoxide lyase pathways of higher plants. Our findings contrast with analyses documented on aboveground barley tissues that mainly emit C6 aldehydes, alcohols and their derivative esters. Moreover, preliminary results indicate quantitative changes in the volatile profile contained in barley roots according to plant age. Multivariate statistical analyses are currently underway to quantitatively assess these changes using plants at five selected developmental stages ranging from germination to the end of tillering.

AB - In chemical ecology, the roles played by root-emitted volatile organic compounds (VOCs) in biotic interactions and the quantitative analysis of such chemicals in root tissues remain poorly documented. In this context, this study aims at developing a fully automated analytical methodology allowing both identification and accurate quantification of VOCs produced by roots of a monocotyledon plant species. Briefly, VOC emitted by crushed barley roots are successively trapped by dynamic headspace sampling on Tenax TA adsorbents, thermally desorbed and cryofocused, separated by gas chromatography (GC) and finally analysed by mass spectrometry (MS) in both SCAN and selected ion monitoring modes. Results show that barley roots mainly produce four volatile aldehydes, namely hexanal, (E)-hex-2-enal, (E)-non-2-enal and (E,Z)-nona-2,6-dienal. These molecules are well-known linoleic (C18:2) and linolenic (C18:3) acid derivatives produced via the lipoxygenase and the hydroperoxide lyase pathways of higher plants. Our findings contrast with analyses documented on aboveground barley tissues that mainly emit C6 aldehydes, alcohols and their derivative esters. Moreover, preliminary results indicate quantitative changes in the volatile profile contained in barley roots according to plant age. Multivariate statistical analyses are currently underway to quantitatively assess these changes using plants at five selected developmental stages ranging from germination to the end of tillering.

KW - Biology

KW - Chemistry

UR - http://www.events.gembloux.ulg.ac.be/nsabs2014/wp-content/uploads/sites/8/2014/02/NSABS2014_book_short_abstracts.pdf

M3 - Published abstract in conference proceedings

SP - 82

BT - Book of short abstracts, Poster presentations

PB - Gembloux Agro-Bio Tech

T2 - 19th National Symposium on Applied Biological Sciences - NSABS 2014

Y2 - 7 February 2014 through 7 February 2014

ER -