Investigations for the detection of genotoxic substances on TLC plates
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In: Journal of Liquid Chromatography and Related Technologies, Vol. 40, No. 2, 20.01.2017, p. 69-74.
Research output: Journal contributions › Journal articles › Research › peer-review
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TY - JOUR
T1 - Investigations for the detection of genotoxic substances on TLC plates
AU - Egetenmeyer, Nicole
AU - Weiss, Stefan C.
PY - 2017/1/20
Y1 - 2017/1/20
N2 - The present study describes modifications to an existing bioautographic method for detecting genotoxic substances on thin-layer chromatography (TLC) plates by using the umu assay. It will be shown that the complex procedure that is described in the literature can be significantly simplified. Using the modified method, it is possible to detect 0.3 ng of the genotoxic substance 4-nitroquinoline-N-oxide. With 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal), a suitable substrate for environmental samples for the detection of the resulting β-galactosidase was found. Experiments showed, in contrast, that indirectly genotoxic substances could not thus far be identified on the TLC plate with overlayer bioautography.
AB - The present study describes modifications to an existing bioautographic method for detecting genotoxic substances on thin-layer chromatography (TLC) plates by using the umu assay. It will be shown that the complex procedure that is described in the literature can be significantly simplified. Using the modified method, it is possible to detect 0.3 ng of the genotoxic substance 4-nitroquinoline-N-oxide. With 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal), a suitable substrate for environmental samples for the detection of the resulting β-galactosidase was found. Experiments showed, in contrast, that indirectly genotoxic substances could not thus far be identified on the TLC plate with overlayer bioautography.
KW - Bioautography
KW - effect-directed analysis
KW - Genotoxicity
KW - HPTLC
KW - Salmonella typhimurium TA1535/pSK 1002
KW - umuC
KW - Biology
UR - http://www.scopus.com/inward/record.url?scp=85015630751&partnerID=8YFLogxK
U2 - 10.1080/10826076.2017.1284674
DO - 10.1080/10826076.2017.1284674
M3 - Journal articles
AN - SCOPUS:85015630751
VL - 40
SP - 69
EP - 74
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
SN - 1082-6076
IS - 2
ER -