Investigations for the detection of genotoxic substances on TLC plates

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Investigations for the detection of genotoxic substances on TLC plates. / Egetenmeyer, Nicole; Weiss, Stefan C.
in: Journal of Liquid Chromatography and Related Technologies, Jahrgang 40, Nr. 2, 20.01.2017, S. 69-74.

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschungbegutachtet

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Egetenmeyer N, Weiss SC. Investigations for the detection of genotoxic substances on TLC plates. Journal of Liquid Chromatography and Related Technologies. 2017 Jan 20;40(2):69-74. doi: 10.1080/10826076.2017.1284674

Bibtex

@article{19a8724f406d471f939bf9d9a37b2cab,
title = "Investigations for the detection of genotoxic substances on TLC plates",
abstract = "The present study describes modifications to an existing bioautographic method for detecting genotoxic substances on thin-layer chromatography (TLC) plates by using the umu assay. It will be shown that the complex procedure that is described in the literature can be significantly simplified. Using the modified method, it is possible to detect 0.3 ng of the genotoxic substance 4-nitroquinoline-N-oxide. With 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal), a suitable substrate for environmental samples for the detection of the resulting β-galactosidase was found. Experiments showed, in contrast, that indirectly genotoxic substances could not thus far be identified on the TLC plate with overlayer bioautography.",
keywords = "Bioautography, effect-directed analysis, Genotoxicity, HPTLC, Salmonella typhimurium TA1535/pSK 1002, umuC, Biology",
author = "Nicole Egetenmeyer and Weiss, {Stefan C.}",
year = "2017",
month = jan,
day = "20",
doi = "10.1080/10826076.2017.1284674",
language = "English",
volume = "40",
pages = "69--74",
journal = "Journal of Liquid Chromatography and Related Technologies",
issn = "1082-6076",
publisher = "Taylor & Francis",
number = "2",

}

RIS

TY - JOUR

T1 - Investigations for the detection of genotoxic substances on TLC plates

AU - Egetenmeyer, Nicole

AU - Weiss, Stefan C.

PY - 2017/1/20

Y1 - 2017/1/20

N2 - The present study describes modifications to an existing bioautographic method for detecting genotoxic substances on thin-layer chromatography (TLC) plates by using the umu assay. It will be shown that the complex procedure that is described in the literature can be significantly simplified. Using the modified method, it is possible to detect 0.3 ng of the genotoxic substance 4-nitroquinoline-N-oxide. With 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal), a suitable substrate for environmental samples for the detection of the resulting β-galactosidase was found. Experiments showed, in contrast, that indirectly genotoxic substances could not thus far be identified on the TLC plate with overlayer bioautography.

AB - The present study describes modifications to an existing bioautographic method for detecting genotoxic substances on thin-layer chromatography (TLC) plates by using the umu assay. It will be shown that the complex procedure that is described in the literature can be significantly simplified. Using the modified method, it is possible to detect 0.3 ng of the genotoxic substance 4-nitroquinoline-N-oxide. With 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal), a suitable substrate for environmental samples for the detection of the resulting β-galactosidase was found. Experiments showed, in contrast, that indirectly genotoxic substances could not thus far be identified on the TLC plate with overlayer bioautography.

KW - Bioautography

KW - effect-directed analysis

KW - Genotoxicity

KW - HPTLC

KW - Salmonella typhimurium TA1535/pSK 1002

KW - umuC

KW - Biology

UR - http://www.scopus.com/inward/record.url?scp=85015630751&partnerID=8YFLogxK

U2 - 10.1080/10826076.2017.1284674

DO - 10.1080/10826076.2017.1284674

M3 - Journal articles

AN - SCOPUS:85015630751

VL - 40

SP - 69

EP - 74

JO - Journal of Liquid Chromatography and Related Technologies

JF - Journal of Liquid Chromatography and Related Technologies

SN - 1082-6076

IS - 2

ER -

DOI