Fluorometer controlled apparatus designed for long-duration algal-feeding experiments and environmental effect studies with mussels
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In: Journal of Marine Biology, Vol. 2013, 401961, 01.01.2013.
Research output: Journal contributions › Journal articles › Research › peer-review
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TY - JOUR
T1 - Fluorometer controlled apparatus designed for long-duration algal-feeding experiments and environmental effect studies with mussels
AU - Pleißner, Daniel
AU - Lundgreen, Kim
AU - Lüskow, Florian
AU - Riisgard, Hans Ulrik
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Experimental feeding and growth studies on filter-feeding organisms often rely on constant algal concentrations maintained over extended periods of time. Here we present a fluorometer controlled apparatus (FCA) designed for feeding experiments with suspension-feeding mussels at naturally low chlorophyll concentrations above 0.5 µg L−1. The principle used is feedback regulation of the algal concentration based on continuous monitoring of the fluorescence intensity of chlorophyll in water pumped through the apparatus from an aquarium with mussels. The filtration rate is monitored continuously as the rate of change of measured volume of an algal stock added to the aquarium for keeping a constant algal concentration. As an example, the FCA has been used to study the filtration rates of blue mussels (Mytilus edulis) at algal concentrations both near and above the incipient saturation level for reduced filtration activity. As another example to put the FCA into perspective as a reliable method for environmental effect studies, the apparatus has been used to demonstrate the acute effect of changing salinity on the filtration rate of M. edulis.
AB - Experimental feeding and growth studies on filter-feeding organisms often rely on constant algal concentrations maintained over extended periods of time. Here we present a fluorometer controlled apparatus (FCA) designed for feeding experiments with suspension-feeding mussels at naturally low chlorophyll concentrations above 0.5 µg L−1. The principle used is feedback regulation of the algal concentration based on continuous monitoring of the fluorescence intensity of chlorophyll in water pumped through the apparatus from an aquarium with mussels. The filtration rate is monitored continuously as the rate of change of measured volume of an algal stock added to the aquarium for keeping a constant algal concentration. As an example, the FCA has been used to study the filtration rates of blue mussels (Mytilus edulis) at algal concentrations both near and above the incipient saturation level for reduced filtration activity. As another example to put the FCA into perspective as a reliable method for environmental effect studies, the apparatus has been used to demonstrate the acute effect of changing salinity on the filtration rate of M. edulis.
KW - Biology
UR - https://www.mendeley.com/catalogue/2253f4cf-3700-37be-a52e-4d6b619396d7/
U2 - 10.1155/2013/401961
DO - 10.1155/2013/401961
M3 - Journal articles
VL - 2013
JO - Journal of Marine Biology
JF - Journal of Marine Biology
SN - 1687-949X
M1 - 401961
ER -