Development of a cell culture system for studying effects of native and photochemically transformed gaseous compounds using an air/liquid culture technique

Research output: Journal contributionsConference abstract in journalResearchpeer-review

Authors

In a series of investigations, an experimental system was established for the study of the interactions of environmentally relevant gaseous compounds and cultured cells. The aim of this study was the development of culture conditions which allow the direct exposure of cells to gaseous compounds at the air/liquid interface for the evaluation of dose-dependent biological effects. The project also aimed at providing the gaseous test substances at concentrations in the sub-vpm region. To achieve these aims, an experimental culture and exposure system was developed which consisted partly of a gas reaction chamber (24001) to provide the possibility of irradiating the gas mixture. The gaseous compounds were conducted to a perspex chamber (3.5 l) in which the cells growing on transwells were exposed. Analysis of the gaseous compounds was performed at the inlet and outlet of the exposure chamber. To assess the cytotoxicity, the following biochemical markers were determined: amount of dsDNA, WST, BrdU, LDH in culture medium and the activity of glutathione-S transferase and esterases. Using this system, dose-dependent cytotoxicity or strong cytotoxic effects were established for ozone (200-900 vpb), PAN (peroxyacetyl nitrate, 20-200 vpb) and NO2 (80-360 vpb). Exposure to purified air did not show significant effects. In addition, some irradiated gas mixtures showed cytotoxicity whereas non-irradiated mixtures did not.

Original languageEnglish
JournalZentralblatt für Hygiene und Umweltmedizin
Volume200
Issue number4
Pages (from-to)402
Number of pages1
ISSN0934-8859
Publication statusPublished - 1997
Externally publishedYes

    Research areas

  • Chemistry - cell culture, chemical reactions, chemicals, cytotoxicity, photochemistry, physical chemistry

Recently viewed

Activities

  1. CyberKant, a Timely Response to the Eclipse of Reason by Mechanical Rationality - 2020
  2. Podiumsdiskussion zu »Heuschrecken«, Rimini Protokoll
  3. Opportunities to Learn, professional beliefs and the Ability to identify Academic Language Features in a Mathematical Explanation. A Study Among Pre-Service Teachers.
  4. User Generated Content
  5. Long-term Internships of Physical Education Pre-service Teachers in the Pandemic: A Mixed-methods Anaylsis
  6. Organized Creativity Virtual Workshop
  7. What tells the driver´s voice about driving situations and driving behaviour?
  8. Games4Health as a new approach to health education
  9. "Listen I‘m sorry. I didn‘t mean to offend you.": Conventions of language use across the Englishes (Universität Erfurt)
  10. Hölderlins philosophische Entwicklung
  11. Leibniz Universität Hannover
  12. VISATT - computergestütze Aufmerksamkeitsanalysen
  13. Erst studieren oder erst probieren: Wie können Core Practices in der Lehrer*innenbildung effektiv vermittelt werden?
  14. Shift Festival - 2010
  15. ABA Antitrust Section Spring Meeting 2001
  16. 7. Konferenz der Tagungsreihe: Between "Total War" and "Small Wars": Studies in the Societal History of the Cold War - 2010
  17. Konferenz - What's the Matter With the Form?
  18. Conference presentation: Integrated Reporting: Current state of empirical research, limitations and future research implications
  19. International Conference on Road Safety and Simulation 2017
  20. International Conference on Renewable Resources and Biorefineries 2017
  21. ARL-Kongress 2012
  22. Advantages and challenges of in silico tools and their contribution to sustainable chemistry with the focus on (Q)SAR
  23. 9. Forum TUMpaed - 2015
  24. Fakultät Management und Technologie (Organisation)
  25. Gutachtertätigkeit für European Association for Research in Learning and Instruction
  26. Einsatz des Transkriptionstools Transana
  27. Albert-Ludwigs-Universität Freiburg (Externe Organisation)
  28. Mathematical Literacy von Eltern in PISA 2003.