A New and Rapid Method for Monitoring the New Oxazolidinone Antibiotic Linezolid in Serum and Urine by High Performance Liquid Chromatography-Integrated Sample Preparation
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A New and Rapid Method for Monitoring the New Oxazolidinone Antibiotic Linezolid in Serum and Urine by High Performance Liquid Chromatography-Integrated Sample Preparation. / Ehrlich, Markus; Trittler, R.; Daschner, Franz D. et al.
In: Journal of Chromatography B, Vol. 755, No. 1-2, 05.05.2001, p. 373-377.Research output: Journal contributions › Journal articles › Research › peer-review
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TY - JOUR
T1 - A New and Rapid Method for Monitoring the New Oxazolidinone Antibiotic Linezolid in Serum and Urine by High Performance Liquid Chromatography-Integrated Sample Preparation
AU - Ehrlich, Markus
AU - Trittler, R.
AU - Daschner, Franz D.
AU - Kümmerer, Klaus
PY - 2001/5/5
Y1 - 2001/5/5
N2 - A sensitive and rapid HPLC-assay for determining the new oxazolidinone antibiotic linezolid in serum and urine is described. HPLC-integrated sample preparation permits the direct injection of serum and urine samples without any pre-treatment. The in-line extraction technique is realized by switching automatically from the extraction column to the analytical column. After the matrix has passed the extraction column the retained analyte will be quantitatively transferred to the analytical column where separation by isocratic HPLC will be performed. Linezolid is detected according to its absorption maximum at 260 nm. The quantification limits are estimated to be 0.3 and 0.5 μg/ml in serum and urine samples, respectively. The described procedure allows sample clean-up and determination of the antibiotic within 20 min, thereby facilitating drug-monitoring in clinical routine.
AB - A sensitive and rapid HPLC-assay for determining the new oxazolidinone antibiotic linezolid in serum and urine is described. HPLC-integrated sample preparation permits the direct injection of serum and urine samples without any pre-treatment. The in-line extraction technique is realized by switching automatically from the extraction column to the analytical column. After the matrix has passed the extraction column the retained analyte will be quantitatively transferred to the analytical column where separation by isocratic HPLC will be performed. Linezolid is detected according to its absorption maximum at 260 nm. The quantification limits are estimated to be 0.3 and 0.5 μg/ml in serum and urine samples, respectively. The described procedure allows sample clean-up and determination of the antibiotic within 20 min, thereby facilitating drug-monitoring in clinical routine.
KW - Linezolid
KW - Oxazolidinones
KW - Chemistry
UR - http://www.scopus.com/inward/record.url?scp=0035810717&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(01)00115-3
DO - 10.1016/S0378-4347(01)00115-3
M3 - Journal articles
VL - 755
SP - 373
EP - 377
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
SN - 1570-0232
IS - 1-2
ER -