A New and Rapid Method for Monitoring the New Oxazolidinone Antibiotic Linezolid in Serum and Urine by High Performance Liquid Chromatography-Integrated Sample Preparation

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A New and Rapid Method for Monitoring the New Oxazolidinone Antibiotic Linezolid in Serum and Urine by High Performance Liquid Chromatography-Integrated Sample Preparation. / Ehrlich, Markus; Trittler, R.; Daschner, Franz D. et al.
In: Journal of Chromatography B, Vol. 755, No. 1-2, 05.05.2001, p. 373-377.

Research output: Journal contributionsJournal articlesResearchpeer-review

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@article{98bc95bf3ec6428eb03dee05e740509a,
title = "A New and Rapid Method for Monitoring the New Oxazolidinone Antibiotic Linezolid in Serum and Urine by High Performance Liquid Chromatography-Integrated Sample Preparation",
abstract = "A sensitive and rapid HPLC-assay for determining the new oxazolidinone antibiotic linezolid in serum and urine is described. HPLC-integrated sample preparation permits the direct injection of serum and urine samples without any pre-treatment. The in-line extraction technique is realized by switching automatically from the extraction column to the analytical column. After the matrix has passed the extraction column the retained analyte will be quantitatively transferred to the analytical column where separation by isocratic HPLC will be performed. Linezolid is detected according to its absorption maximum at 260 nm. The quantification limits are estimated to be 0.3 and 0.5 μg/ml in serum and urine samples, respectively. The described procedure allows sample clean-up and determination of the antibiotic within 20 min, thereby facilitating drug-monitoring in clinical routine.",
keywords = "Linezolid, Oxazolidinones, Chemistry",
author = "Markus Ehrlich and R. Trittler and Daschner, {Franz D.} and Klaus K{\"u}mmerer",
year = "2001",
month = may,
day = "5",
doi = "10.1016/S0378-4347(01)00115-3",
language = "English",
volume = "755",
pages = "373--377",
journal = "Journal of Chromatography B",
issn = "1873-376X",
publisher = "Elsevier B.V.",
number = "1-2",

}

RIS

TY - JOUR

T1 - A New and Rapid Method for Monitoring the New Oxazolidinone Antibiotic Linezolid in Serum and Urine by High Performance Liquid Chromatography-Integrated Sample Preparation

AU - Ehrlich, Markus

AU - Trittler, R.

AU - Daschner, Franz D.

AU - Kümmerer, Klaus

PY - 2001/5/5

Y1 - 2001/5/5

N2 - A sensitive and rapid HPLC-assay for determining the new oxazolidinone antibiotic linezolid in serum and urine is described. HPLC-integrated sample preparation permits the direct injection of serum and urine samples without any pre-treatment. The in-line extraction technique is realized by switching automatically from the extraction column to the analytical column. After the matrix has passed the extraction column the retained analyte will be quantitatively transferred to the analytical column where separation by isocratic HPLC will be performed. Linezolid is detected according to its absorption maximum at 260 nm. The quantification limits are estimated to be 0.3 and 0.5 μg/ml in serum and urine samples, respectively. The described procedure allows sample clean-up and determination of the antibiotic within 20 min, thereby facilitating drug-monitoring in clinical routine.

AB - A sensitive and rapid HPLC-assay for determining the new oxazolidinone antibiotic linezolid in serum and urine is described. HPLC-integrated sample preparation permits the direct injection of serum and urine samples without any pre-treatment. The in-line extraction technique is realized by switching automatically from the extraction column to the analytical column. After the matrix has passed the extraction column the retained analyte will be quantitatively transferred to the analytical column where separation by isocratic HPLC will be performed. Linezolid is detected according to its absorption maximum at 260 nm. The quantification limits are estimated to be 0.3 and 0.5 μg/ml in serum and urine samples, respectively. The described procedure allows sample clean-up and determination of the antibiotic within 20 min, thereby facilitating drug-monitoring in clinical routine.

KW - Linezolid

KW - Oxazolidinones

KW - Chemistry

UR - http://www.scopus.com/inward/record.url?scp=0035810717&partnerID=8YFLogxK

U2 - 10.1016/S0378-4347(01)00115-3

DO - 10.1016/S0378-4347(01)00115-3

M3 - Journal articles

VL - 755

SP - 373

EP - 377

JO - Journal of Chromatography B

JF - Journal of Chromatography B

SN - 1873-376X

IS - 1-2

ER -