TY - JOUR

T1 - Improved cytotoxicity testing of magnesium materials

AU - Fischer, Janine

AU - Pröfrock, Daniel

AU - Hort, Norbert

AU - Willumeit, Regine

AU - Feyerabend, Frank

PY - 2011/6/25

Y1 - 2011/6/25

N2 - Metallic magnesium (Mg) and its alloys are highly suitable for medical applications as biocompatible and biodegradable implant materials. Magnesium has mechanical properties similar to bone, stimulates bone regeneration, is an essential non-toxic element for the human body and degrades completely within the body environment. In consequence, magnesium is a promising candidate as implant material for orthopaedic applications. Protocols using the guideline of current ISO standards should be carefully evaluated when applying them for the characterization of the cytotoxic potential of degradable magnesium materials. For as-cast material we recommend using 10 times more extraction medium than recommended by the ISO standards to obtain reasonable results for reliable cytotoxicity rankings of degradable materials in vitro. In addition primary isolated human osteoblasts or mesenchymal stem cells should be used to test magnesium materials.

AB - Metallic magnesium (Mg) and its alloys are highly suitable for medical applications as biocompatible and biodegradable implant materials. Magnesium has mechanical properties similar to bone, stimulates bone regeneration, is an essential non-toxic element for the human body and degrades completely within the body environment. In consequence, magnesium is a promising candidate as implant material for orthopaedic applications. Protocols using the guideline of current ISO standards should be carefully evaluated when applying them for the characterization of the cytotoxic potential of degradable magnesium materials. For as-cast material we recommend using 10 times more extraction medium than recommended by the ISO standards to obtain reasonable results for reliable cytotoxicity rankings of degradable materials in vitro. In addition primary isolated human osteoblasts or mesenchymal stem cells should be used to test magnesium materials.

KW - Cytotoxicity tests

KW - Implants

KW - In vitro

KW - ISO standards

KW - Magnesium

KW - Osmolality

KW - Engineering

UR - http://www.scopus.com/inward/record.url?scp=79957674339&partnerID=8YFLogxK

U2 - 10.1016/j.mseb.2011.04.008

DO - 10.1016/j.mseb.2011.04.008

M3 - Journal articles

AN - SCOPUS:79957674339

VL - 176

SP - 830

EP - 834

JO - Materials Science and Engineering: B

JF - Materials Science and Engineering: B

SN - 0921-5107

IS - 11

ER -