TY - JOUR

T1 - Heterotrophic growth of Galdieria sulphuraria on residues from aquaculture and fish processing industries

AU - Pleissner, Daniel

AU - Schönfelder, Stephanie

AU - Händel, Nicole

AU - Dalichow, Julia

AU - Ettinger, Judith

AU - Kvangarsnes, Kristine

AU - Dauksas, Egidijus

AU - Rustad, Turid

AU - Cropotova, Janna

N1 - This work was financially supported by the German Federal Ministry of Food and Agriculture /BMEL) through the Federal Office for Agriculture and Food /BLE), grant number 2821ERA12C. This project has received funding from the European Union’s Horizon 2020 research and innovation program under the grant agreement No 862555. Janna Cropotova acknowledges the FOSC-ERA project “CLIMAQUA”, (grant No. 327695 of BIONÆR-Bionæringsprogram) for support. Publisher Copyright: © 2023 The Author(s)

PY - 2023/9/1

Y1 - 2023/9/1

N2 - The study aimed at zero-waste utilization of fish processing streams for cultivation of microalgae Galdieria sulphuraria. Wastewater from a fish processing facility, slam (mix of used fish feed and faeces), and dried pellet (sediments after enzymatic hydrolysis of rainbow trout) were investigated as potential sources of carbon, nitrogen, and phosphate for cultivation of G. sulphuraria. The pellet extract was found to support the growth of G. sulphuraria when appropriate diluted, at concentrations below 40 % (v/v). It was revealed that wastewater does not impact the growth negatively, however free amino nitrogen and carbon sources need to be supplied from another source. Therefore, only proteolyzed pellet extract (20 %, v/v) was selected for upscaling and a biomass concentration of 80 g L−1 (growth rate was 0.72 day−1) was achieved in a non-sterile fed-batch culture. Even though biomass was produced under non-sterile conditions no pathogens such as Salmonella sp. could be detected.

AB - The study aimed at zero-waste utilization of fish processing streams for cultivation of microalgae Galdieria sulphuraria. Wastewater from a fish processing facility, slam (mix of used fish feed and faeces), and dried pellet (sediments after enzymatic hydrolysis of rainbow trout) were investigated as potential sources of carbon, nitrogen, and phosphate for cultivation of G. sulphuraria. The pellet extract was found to support the growth of G. sulphuraria when appropriate diluted, at concentrations below 40 % (v/v). It was revealed that wastewater does not impact the growth negatively, however free amino nitrogen and carbon sources need to be supplied from another source. Therefore, only proteolyzed pellet extract (20 %, v/v) was selected for upscaling and a biomass concentration of 80 g L−1 (growth rate was 0.72 day−1) was achieved in a non-sterile fed-batch culture. Even though biomass was produced under non-sterile conditions no pathogens such as Salmonella sp. could be detected.

KW - Algal biomass

KW - Bioeconomy

KW - Bioprocess

KW - Fish processing

KW - Protein residues

KW - Biology

UR - http://www.scopus.com/inward/record.url?scp=85162136531&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2023.129281

DO - 10.1016/j.biortech.2023.129281

M3 - Journal articles

C2 - 37295476

AN - SCOPUS:85162136531

VL - 384

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

M1 - 129281

ER -