Development and characterisation of a new interface for coupling capillary LC with collision-cell ICPMS and its application for phosphorylation profiling of tryptic protein digests

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschungbegutachtet

Standard

Development and characterisation of a new interface for coupling capillary LC with collision-cell ICPMS and its application for phosphorylation profiling of tryptic protein digests. / Profrock, Daniel; Leonhard, P; Ruck, W et al.
in: Analytical & Bioanalytical Chemistry, Jahrgang 381, Nr. 1, 01.2005, S. 194-204.

Publikation: Beiträge in ZeitschriftenZeitschriftenaufsätzeForschungbegutachtet

Harvard

APA

Vancouver

Bibtex

@article{ef184b5bd103448ab24355f4a8778ba0,
title = "Development and characterisation of a new interface for coupling capillary LC with collision-cell ICPMS and its application for phosphorylation profiling of tryptic protein digests",
abstract = "A comparison of different nebulisers for direct hyphenation of capillary and nano liquid chromatography (Cap-LC, Nano-LC) and quadrupole-based collision cell inductively coupled plasma mass spectrometry (CC-ICP-MS) for phosphorylation profiling of tryptic protein digests is described. Helium was used as cell gas and specially tuned instrumental conditions were used to achieve background minimisation at the mass of phosphorus, because of kinetic energy discrimination of the interfering polyatomic ions. The proposed set-up is based on a modified capillary electrophoresis interface and a home-made 4-mL spray chamber. It enables the use of gradient conditions with a highly concentrated organic mobile phase as often used in protein phosphorylation analysis, without the need to apply membrane desolvation for removal of the organic phase or further background minimisation. No significant signal suppression or other negative effects caused by the organic mobile phase occur, because of the low flow rates used in Cap-LC and the robust plasma conditions of the CC-ICP-MS instrument. A tryptic digest of beta-casein was investigated as model compound to demonstrate the applicability of the proposed set-up for phosphorylation profiling in protein analysis using quadrupole based collision-cell ICP-MS as phosphorus-specific detector. Detection limits for phosphorylated peptides down to the sub picomole level were obtained. As a complementary technique, electrospray ionisation tandem mass spectrometry (ESI-MS-MS) with data base searching was used for further characterisation of the phosphorylated peptides detected.",
keywords = "Chemistry, Capillary-LC, Protein, Phosphorylation , Post-translational modification , ICP–MS, Hyphenation , Collision cell, Reaction cell",
author = "Daniel Profrock and P Leonhard and W Ruck and A Prange",
year = "2005",
month = jan,
doi = "10.1007/s00216-004-2930-5",
language = "English",
volume = "381",
pages = "194--204",
journal = "Analytical & Bioanalytical Chemistry",
issn = "1618-2642",
publisher = "Springer Science and Business Media Deutschland",
number = "1",

}

RIS

TY - JOUR

T1 - Development and characterisation of a new interface for coupling capillary LC with collision-cell ICPMS and its application for phosphorylation profiling of tryptic protein digests

AU - Profrock, Daniel

AU - Leonhard, P

AU - Ruck, W

AU - Prange, A

PY - 2005/1

Y1 - 2005/1

N2 - A comparison of different nebulisers for direct hyphenation of capillary and nano liquid chromatography (Cap-LC, Nano-LC) and quadrupole-based collision cell inductively coupled plasma mass spectrometry (CC-ICP-MS) for phosphorylation profiling of tryptic protein digests is described. Helium was used as cell gas and specially tuned instrumental conditions were used to achieve background minimisation at the mass of phosphorus, because of kinetic energy discrimination of the interfering polyatomic ions. The proposed set-up is based on a modified capillary electrophoresis interface and a home-made 4-mL spray chamber. It enables the use of gradient conditions with a highly concentrated organic mobile phase as often used in protein phosphorylation analysis, without the need to apply membrane desolvation for removal of the organic phase or further background minimisation. No significant signal suppression or other negative effects caused by the organic mobile phase occur, because of the low flow rates used in Cap-LC and the robust plasma conditions of the CC-ICP-MS instrument. A tryptic digest of beta-casein was investigated as model compound to demonstrate the applicability of the proposed set-up for phosphorylation profiling in protein analysis using quadrupole based collision-cell ICP-MS as phosphorus-specific detector. Detection limits for phosphorylated peptides down to the sub picomole level were obtained. As a complementary technique, electrospray ionisation tandem mass spectrometry (ESI-MS-MS) with data base searching was used for further characterisation of the phosphorylated peptides detected.

AB - A comparison of different nebulisers for direct hyphenation of capillary and nano liquid chromatography (Cap-LC, Nano-LC) and quadrupole-based collision cell inductively coupled plasma mass spectrometry (CC-ICP-MS) for phosphorylation profiling of tryptic protein digests is described. Helium was used as cell gas and specially tuned instrumental conditions were used to achieve background minimisation at the mass of phosphorus, because of kinetic energy discrimination of the interfering polyatomic ions. The proposed set-up is based on a modified capillary electrophoresis interface and a home-made 4-mL spray chamber. It enables the use of gradient conditions with a highly concentrated organic mobile phase as often used in protein phosphorylation analysis, without the need to apply membrane desolvation for removal of the organic phase or further background minimisation. No significant signal suppression or other negative effects caused by the organic mobile phase occur, because of the low flow rates used in Cap-LC and the robust plasma conditions of the CC-ICP-MS instrument. A tryptic digest of beta-casein was investigated as model compound to demonstrate the applicability of the proposed set-up for phosphorylation profiling in protein analysis using quadrupole based collision-cell ICP-MS as phosphorus-specific detector. Detection limits for phosphorylated peptides down to the sub picomole level were obtained. As a complementary technique, electrospray ionisation tandem mass spectrometry (ESI-MS-MS) with data base searching was used for further characterisation of the phosphorylated peptides detected.

KW - Chemistry

KW - Capillary-LC

KW - Protein

KW - Phosphorylation

KW - Post-translational modification

KW - ICP–MS

KW - Hyphenation

KW - Collision cell

KW - Reaction cell

UR - http://www.scopus.com/inward/record.url?scp=13844267041&partnerID=8YFLogxK

U2 - 10.1007/s00216-004-2930-5

DO - 10.1007/s00216-004-2930-5

M3 - Journal articles

C2 - 15592818

VL - 381

SP - 194

EP - 204

JO - Analytical & Bioanalytical Chemistry

JF - Analytical & Bioanalytical Chemistry

SN - 1618-2642

IS - 1

ER -

DOI

Zuletzt angesehen

Publikationen

  1. Integrating regional perceptions into climate change adaptation
  2. Value-based management in banking
  3. Feel the Music! Exploring the Cross-modal Correspondence between Music and Haptic Perceptions of Softness
  4. Structural ambidexterity, transition processes, and integration trade‐offs: a longitudinal study of failed exploration
  5. How and where to map supply and demand of ecosystem services for policy-relevant outcomes?
  6. The promise and Pitfalls of a blended, video- and coaching-based professional development program in Germany
  7. E-Mail als sozio-technisches Hybrid
  8. The Use of Environmental Management Accounting for Investment in and Control of ‘Clean Development Mechanism’ Projects
  9. Continuous Casting with Mid-Process Alloying
  10. A Kalman estimator for detecting repetitive disturbances
  11. Collaborative Information Systems zur Selbstorganisation von ad-hoc-Helfern
  12. Transformationsnarrative
  13. Support from the Internet for Individuals with Mental Disorders
  14. Implementing inquiry-based science education to foster emotional engagement of special-needs students
  15. Einführung in Grundlagen der theoretischen Informatik
  16. Estimation of physicochemical properties of 52 non-PBDE brominated flame retardants and evaluation of their overall persistence and long-range transport potential
  17. A Fictional Risk Narrative and Its Potential for Social Resonance: Reception of Barbara Kingsolver’s Flight Behavior in Reviews and Reading Groups
  18. Towards an Intra- and Interorganizational Perspective
  19. Determinants of Credit Allocation for Photovoltaic Projects
  20. Pragmatic Function of Twitter Handlers' Perspectives on Children Discourse in Nigeria.
  21. Pragmatic Competence in EIL
  22. Lernwerkstatt
  23. Selecting methods for ecosystem service assessment
  24. Performativität
  25. The impact of goal specificity and goal type on learning outcome and cognitive load