Blue-green light is required for a maximized fatty acid unsaturation and pigment concentration in the microalga Acutodesmus obliquus
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Blue-green light is required for a maximized fatty acid unsaturation and pigment concentration in the microalga Acutodesmus obliquus. / Helamieh, Mark; Reich, Marco; Bory, Sophie et al.
in: Lipids, Jahrgang 57, Nr. 4-5, 01.07.2022, S. 221-232.Publikation: Beiträge in Zeitschriften › Zeitschriftenaufsätze › Forschung › begutachtet
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T1 - Blue-green light is required for a maximized fatty acid unsaturation and pigment concentration in the microalga Acutodesmus obliquus
AU - Helamieh, Mark
AU - Reich, Marco
AU - Bory, Sophie
AU - Rohne, Philipp
AU - Riebesell, Ulf
AU - Kerner, Martin
AU - Kümmerer, Klaus
N1 - Publisher Copyright: © 2022 The Authors. Lipids published by Wiley Periodicals LLC on behalf of AOCS.
PY - 2022/7/1
Y1 - 2022/7/1
N2 - Blue-green light is known to maximize the degree of fatty acid (FA) unsaturation in microalgae. However, knowledge on the particular waveband responsible for this stimulation of FA desaturation and its impact on the pigment composition in microalgae remains limited. In this study, Acutodesmus obliquus was cultivated for 96 h at 15°C with different light spectra (380–700 nm, 470–700 nm, 520–700 nm, 600–700 nm, and dark controls). Growth was monitored daily, and qualitative characterization of the microalgal FA composition was achieved via gas chromatography coupled with electron impact ionization mass spectrometry (GC-EI/MS). Additionally, a quantitative analysis of microalgal pigments was performed using high-performance liquid chromatography with diode array detection (HPLC-DAD). Spectra that included wavelengths between 470 and 520 nm led to a significantly higher percentage of the polyunsaturated fatty acids (PUFA) 18:3 and 16:4, compared to all other light conditions. However, no significant differences between the red light cultivations and the heterotrophic dark controls were observed for the FA 18:3 and 16:4. These results indicate, that exclusively the blue-green light waveband between 470 and 520 nm is responsible for a maximized FA unsaturation in A. obliquus. Furthermore, the growth and production of pigments were impaired if blue-green light (380–520 nm) was absent in the light spectrum. This knowledge can contribute to achieving a suitable microalgal pigment and FA composition for industrial purposes and must be considered in spectrally selective microalgae cultivation systems.
AB - Blue-green light is known to maximize the degree of fatty acid (FA) unsaturation in microalgae. However, knowledge on the particular waveband responsible for this stimulation of FA desaturation and its impact on the pigment composition in microalgae remains limited. In this study, Acutodesmus obliquus was cultivated for 96 h at 15°C with different light spectra (380–700 nm, 470–700 nm, 520–700 nm, 600–700 nm, and dark controls). Growth was monitored daily, and qualitative characterization of the microalgal FA composition was achieved via gas chromatography coupled with electron impact ionization mass spectrometry (GC-EI/MS). Additionally, a quantitative analysis of microalgal pigments was performed using high-performance liquid chromatography with diode array detection (HPLC-DAD). Spectra that included wavelengths between 470 and 520 nm led to a significantly higher percentage of the polyunsaturated fatty acids (PUFA) 18:3 and 16:4, compared to all other light conditions. However, no significant differences between the red light cultivations and the heterotrophic dark controls were observed for the FA 18:3 and 16:4. These results indicate, that exclusively the blue-green light waveband between 470 and 520 nm is responsible for a maximized FA unsaturation in A. obliquus. Furthermore, the growth and production of pigments were impaired if blue-green light (380–520 nm) was absent in the light spectrum. This knowledge can contribute to achieving a suitable microalgal pigment and FA composition for industrial purposes and must be considered in spectrally selective microalgae cultivation systems.
KW - blue-green light
KW - fatty acid
KW - lutein
KW - microalgae
KW - photosynthetic pigment
KW - Chemistry
UR - http://www.scopus.com/inward/record.url?scp=85128785353&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/16e9e449-2e34-32eb-89ec-5cf73d5539dd/
U2 - 10.1002/lipd.12343
DO - 10.1002/lipd.12343
M3 - Journal articles
C2 - 35460080
AN - SCOPUS:85128785353
VL - 57
SP - 221
EP - 232
JO - Lipids
JF - Lipids
SN - 0024-4201
IS - 4-5
ER -